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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 2950-3000 of 96812 product results
Myelin oligodendrocyte glycoprotein/MOG, Polyclonal Antibody (Cat# AAA124951)
Full Name
Anti-Myelin oligodendrocyte glycoprotein/MOG Antibody
Gene Names
MOG; BTN6; BTNL11; MOGIG2; NRCLP7
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of P cadherin using anti-P cadherin antibody.P cadherin was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-P cadherin antibody.P cadherin was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-P cadherin antibody.P cadherin was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-P cadherin antibody.P cadherin was detected in paraffin-embedded section of human cholangiocarcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-P cadherin antibody.P cadherin was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-P cadherin antibody.P cadherin was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-P cadherin Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
P cadherin/CDH3, Polyclonal Antibody (Cat# AAA124952)
Full Name
Anti-P cadherin/CDH3 Antibody
Gene Names
CDH3; CDHP; HJMD; PCAD
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
SLC34A2, Polyclonal Antibody (Cat# AAA124955)
Full Name
Anti-SLC34A2 Antibody
Gene Names
SLC34A2; NPTIIb; NAPI-3B; NAPI-IIb
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of HAS1 using anti- HAS1 antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human SHG-44 whole cell lysates,Lane 2: human THP-1 whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: rat smooth muscle tissue lysates,Lane 5: rat ovary tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse smooth muscle tissue lysates,Lane 8: mouse ovary tissue lysates,Lane 9: mouse small intestine tissue lysates,Lane 10: mouse Neuro-2a whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- HAS1 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HAS1 at approximately 70KD. The expected band size for HAS1 is at 65KD.)
Hyaluronan synthase 1/HAS1, Polyclonal Antibody (Cat# AAA124958)
Full Name
Anti-Hyaluronan synthase 1/HAS1 Antibody
Gene Names
HAS1; HAS
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of Cathepsin E using anti-Cathepsin E antibody.Cathepsin E was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-Cathepsin E antibody.Cathepsin E was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-Cathepsin E Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Cathepsin E/CTSE, Polyclonal Antibody (Cat# AAA124960)
Full Name
Anti-Cathepsin E/CTSE Antibody
Gene Names
CTSE; CATE
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of FMN1 using anti-FMN1 antibody (AAA124738).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysate,Lane 2: mouse brain tissue lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMN1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FMN1 at approximately 158KD. The expected band size for FMN1 is at 158KD.)
FMN1, Polyclonal Antibody (Cat# AAA124738)
Full Name
Anti-FMN1 Picoband Antibody
Gene Names
FMN1; LD; FMN
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of Relaxin 1 using anti-Relaxin 1 antibody (AAA124740).Relaxin 1 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Relaxin 1 Antibody (AAA124740) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Relaxin 1, Polyclonal Antibody (Cat# AAA124740)
Full Name
Anti-Relaxin 1 Picoband Antibody
Gene Names
RLN1; H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
Reactivity
Human, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of Connexin 45/GJA7 using anti- Connexin 45/GJA7 antibody (AAA124741).Connexin 45/GJA7 was detected in paraffin-embedded section of human placneta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Connexin 45/GJA7 Antibody (AAA124741) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Connexin 45/GJA7, Polyclonal Antibody (Cat# AAA124741)
Full Name
Anti-Connexin 45/GJA7 Picoband Antibody
Gene Names
GJC1; CX45; GJA7
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of Alpha Amylase 1 using anti- Alpha Amylase 1 antibody (AAA124745).Alpha Amylase 1 was detected in paraffin-embedded section of human pancreatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Alpha Amylase 1 Antibody (AAA124745) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Alpha Amylase 1, Polyclonal Antibody (Cat# AAA124745)
Full Name
Anti-Alpha Amylase 1 Picoband Antibody
Gene Names
AMY1B; AMY1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(Western blot analysis of SLC25A37 expression in Human THP-1. SLC25A37 at 37KD was detected using rabbit anti-SLC25A37 Antigen Affinity purified polyclonal antibody at Dilution: 0.2-1ug/ml. The blot was developed using chemiluminescence (ECL) method)
SLC25A37/Mitoferrin, Polyclonal Antibody (Cat# AAA124885)
Full Name
Anti-SLC25A37/Mitoferrin Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of HP using anti-HP antibod.)
Haptoglobin/HP, Polyclonal Antibody (Cat# AAA124887)
Full Name
Anti-Haptoglobin/HP Antibody
Gene Names
HP; BP; HPA1S; HP2ALPHA2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of C99 using anti-C99 antibody.)
APP/C99, Polyclonal Antibody (Cat# AAA124888)
Full Name
Anti-APP/C99 Antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of AHR using anti-AHR antibody.AHR was detected in paraffin-embedded section of human cholangiocarcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHR antibody.AHR was detected in paraffin-embedded section of human cholangiocarcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHR antibody.AHR was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHR antibody.AHR was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHR antibody.AHR was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHR antibody.AHR was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHR antibody.AHR was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHR antibody.Overlay histogram showing U87 cells stained with A00225-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AHR antibody.Overlay histogram showing U937 cells stained with A00225-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AHR Antibody for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
AHR, Polyclonal Antibody (Cat# AAA124893)
Full Name
Anti-AHR Antibody
Gene Names
AHR; bHLHe76
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of Cdk6 using anti-Cdk6 antibody.Cdk6 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-Cdk6 antibody.Cdk6 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-Cdk6 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Cdk6, Polyclonal Antibody (Cat# AAA124898)
Full Name
Anti-Cdk6 Antibody
Gene Names
CDK6; MCPH12; PLSTIRE
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Figure 1. IHC analysis of CIC using anti-CIC antibody.CIC was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CIC Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CIC, Polyclonal Antibody (Cat# AAA124900)
Full Name
Anti-CIC Antibody
Gene Names
CIC; MRD45
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of TANK using anti-TANK antibody.TANK was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TANK antibody.TANK was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TANK antibody.TANK was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TANK antibody.TANK was detected in paraffin-embedded section of human cholangiocarcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TANK antibody.TANK was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TANK antibody.TANK was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TANK Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
TANK, Polyclonal Antibody (Cat# AAA124902)
Full Name
Anti-TANK Antibody
Gene Names
TANK; ITRAF; TRAF2; I-TRAF
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of MDC1 using anti-MDC1 antibody (AAA124633).MDC1 was detected in paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MDC1 Antibody (AAA124633) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
MDC1, Polyclonal Antibody (Cat# AAA124633)
Full Name
Anti-MDC1 antibody
Gene Names
MDC1; NFBD1
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Immunohistochemistry
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of RXRA using anti-RXRA antibody (AAA124639).RXRA was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RXRA Antibody (AAA124639) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
RXRA/Rxr Alpha, Polyclonal Antibody (Cat# AAA124639)
Full Name
Anti-RXRA/Rxr Alpha Picoband antibody
Gene Names
RXRA; NR2B1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of PC-3 cells using anti-SATB1 antibody (AAA124640).Overlay histogram showing PC-3 cells stained with AAA124640 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SATB1 Antibody (AAA124640,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SATB1, Polyclonal Antibody (Cat# AAA124640)
Full Name
Anti-SATB1 Picoband antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of CACO-2 cells using anti-IDE antibody (AAA124641).Overlay histogram showing CACO-2 cells stained with AAA124641 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDE Antibody (AAA124641,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IDE, Polyclonal Antibody (Cat# AAA124641)
Full Name
Anti-IDE Picoband antibody
Gene Names
IDE; INSULYSIN
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Pricing
WB (Western Blot)
(Figure 2. Western blot analysis of DNA Polymerase iota using anti-DNA Polymerase iota antibody (AAA124644).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat testis tissue lysates,Lane 2: rat testis tissue lysates,Lane 3: rat kidney tissue lysates,Lane 4: rat stomach tissue lysates,Lane 5: mouse testis tissue lysates,Lane 6: mouse testis tissue lysates,Lane 7: mouse kidney tissue lysates,Lane 8: mouse stomach tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Polymerase iota antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DNA Polymerase iota at approximately 83KD. The expected band size for DNA Polymerase iota is at 83KD.)
DNA Polymerase iota, Polyclonal Antibody (Cat# AAA124644)
Full Name
Anti-DNA Polymerase iota Picoband antibody
Gene Names
POLI; eta2; RAD30B; RAD3OB
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U251 cells using anti-NRXN1 antibody (AAA124650).Overlay histogram showing U251 cells stained with AAA124650 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRXN1 Antibody (AAA124650,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Neurexin 1, Polyclonal Antibody (Cat# AAA124650)
Full Name
Anti-Neurexin 1 antibody
Gene Names
NRXN1; PTHSL2; SCZD17; Hs.22998
Reactivity
Human
Applications
Western Blot, Flow Cytometry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of CKB using anti-CKB antibody (AAA124662).CKB was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CKB Antibody (AAA124662) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CKB, Polyclonal Antibody (Cat# AAA124662)
Full Name
Anti-CKB Picoband antibody
Gene Names
CKB; BCK; B-CK; CKBB; HEL-211; HEL-S-29
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of TFF3 using anti-TFF3 antibody (AAA124664).TFF3 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TFF3 Antibody (AAA124664) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
TFF3/Itf, Polyclonal Antibody (Cat# AAA124664)
Full Name
Anti-TFF3/Itf Picoband antibody
Gene Names
Tff3; ITF; mITF
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of RAW264.7 cells using anti-CCR3 antibody (AAA124666).Overlay histogram showing RAW264.7 cells stained with AAA124666 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCR3 Antibody (AAA124666,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CCR3, Polyclonal Antibody (Cat# AAA124666)
Full Name
Anti-CCR3 Picoband antibody
Gene Names
CCR3; CKR3; CD193; CMKBR3; CC-CKR-3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of RUNX1T1/ETO using anti-RUNX1T1/ETO antibody (AAA124670).RUNX1T1/ETO was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RUNX1T1/ETO Antibody (AAA124670) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
RUNX1T1/ETO, Polyclonal Antibody (Cat# AAA124670)
Full Name
Anti-RUNX1T1/ETO antibody
Gene Names
RUNX1T1; CDR; ETO; MTG8; AML1T1; ZMYND2; CBFA2T1; AML1-MTG8
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Immunocytochemistry, Western Blot, Immunohistochemistry
Purity
Immunogoen affinity purified.
Pricing
Application Data
POFUT1, Polyclonal Antibody (Cat# AAA112104)
Full Name
POFUT1 Antibody
Gene Names
POFUT1; DDD2; FUT12; O-FUT; OFUCT1; O-Fuc-T; O-FucT-1
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
PLEKHG6, Polyclonal Antibody (Cat# AAA112110)
Full Name
PLEKHG6 Antibody
Gene Names
PLEKHG6; MyoGEF
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
TARBP2, Polyclonal Antibody (Cat# AAA112117)
Full Name
TARBP2 Antibody
Gene Names
TARBP2; LOQS; TRBP; TRBP1; TRBP2
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
BRD7, Polyclonal Antibody (Cat# AAA112129)
Full Name
BRD7 Antibody
Gene Names
BRD7; BP75; NAG4; CELTIX1
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
DDX20, Polyclonal Antibody (Cat# AAA112133)
Full Name
DDX20 Antibody
Gene Names
DDX20; DP103; GEMIN3
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
OSBPL10, Polyclonal Antibody (Cat# AAA112141)
Full Name
OSBPL10 Antibody
Gene Names
OSBPL10; ORP10; OSBP9
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
ASTN2, Polyclonal Antibody (Cat# AAA112153)
Full Name
ASTN2 Antibody
Gene Names
ASTN2; bA67K19.1
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
ARHGDIG, Polyclonal Antibody (Cat# AAA112157)
Full Name
ARHGDIG Antibody
Gene Names
ARHGDIG; RHOGDI-3
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
APEH, Polyclonal Antibody (Cat# AAA112161)
Full Name
APEH Antibody
Gene Names
APEH; APH; OPH; AARE; ACPH; D3S48E; D3F15S2; DNF15S2
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
ZNF560, Polyclonal Antibody (Cat# AAA112169)
Full Name
ZNF560 Antibody
Gene Names
NFKBID; TA-NFKBH; IkappaBNS
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Western Blot
Pricing
Application Data
NGFR, Polyclonal Antibody (Cat# AAA112181)
Full Name
NGFR Antibody
Gene Names
NGFR; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
ZNF471, Polyclonal Antibody (Cat# AAA112185)
Full Name
ZNF471 Antibody
Gene Names
ZNF471; ERP1; Z1971
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
PPM1D, Polyclonal Antibody (Cat# AAA111468)
Full Name
PPM1D Antibody
Gene Names
PPM1D; WIP1; IDDGIP; PP2C-DELTA
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
POLB, Polyclonal Antibody (Cat# AAA111473)
Full Name
POLB Antibody
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
GRB7, Polyclonal Antibody (Cat# AAA111484)
Full Name
GRB7 Antibody
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
OSGEPL1, Polyclonal Antibody (Cat# AAA111489)
Full Name
OSGEPL1 Antibody
Gene Names
OSGEPL1; Qri7; OSGEPL
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
TDGF1, Polyclonal Antibody (Cat# AAA111490)
Full Name
TDGF1 Antibody
Gene Names
TDGF1; CR; CRGF; CRIPTO
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
PMPCA, Polyclonal Antibody (Cat# AAA111499)
Full Name
PMPCA Antibody
Gene Names
PMPCA; CLA1; CPD3; P-55; SCAR2; INPP5E; Alpha-MPP
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
ZNF606, Polyclonal Antibody (Cat# AAA111500)
Full Name
ZNF606 Antibody
Gene Names
ZNF606; ZNF328
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
FIS1, Polyclonal Antibody (Cat# AAA111508)
Full Name
FIS1 Antibody
Gene Names
FIS1; TTC11; CGI-135
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
POLDIP3, Polyclonal Antibody (Cat# AAA111515)
Full Name
POLDIP3 Antibody
Gene Names
POLDIP3; SKAR; PDIP3; PDIP46
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
EARS2, Polyclonal Antibody (Cat# AAA112030)
Full Name
EARS2 Antibody
Gene Names
EARS2; MSE1; gluRS; COXPD12
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
FLT3, Polyclonal Antibody (Cat# AAA112032)
Full Name
FLT3 Antibody
Gene Names
FLT3; FLK2; STK1; CD135; FLK-2
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot, Peptide ELISA
Pricing
Application Data
ACVR2B, Polyclonal Antibody (Cat# AAA112034)
Full Name
ACVR2B Antibody
Gene Names
ACVR2B; HTX4; ACTRIIB; ActR-IIB
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. This ability to bind multiple epitopes is what makes polyclonal antibodies highly sensitive, as explained in our detailed guide on polyclonal antibodies and why they matter.
As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in our catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.