IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APH1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit APH1 Polyclonal Antibody | anti-APH1A antibody
APH1 Antibody (N-term)
Gene Names
APH1A; APH-1; APH-1A; CGI-78; 6530402N02Rik
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
APH1, Antibody; APH1 Antibody (N-term); Gamma-secretase subunit APH-1A; APH-1a; Aph-1alpha; Presenilin-stabilization factor; APH1A; PSF; anti-APH1A antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This APH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-109 amino acids from the N-terminal region of human APH1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
77-109
Sequence Length
195
Applicable Applications for anti-APH1A antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APH1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APH1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-APH1 Antibody (N-term) in A2058 cell line lysates (35ug/lane). APH1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-APH1 Antibody (N-term) in A2058 cell line lysates (35ug/lane). APH1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-APH1A antibody
APH1 is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). The gamma-secretase complex is minimally composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2, although other components may exist. APH1 probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
Product Categories/Family for anti-APH1A antibody
References
Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
Marlow, L., et al., Biochem. Biophys. Res. Commun. 305(3):502-509 (2003).
Kimberly, W.T., et al., Proc. Natl. Acad. Sci. U.S.A. 100(11):6382-6387 (2003).
Edbauer, D., et al., Nat. Cell Biol. 5(5):486-488 (2003).
Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
Marlow, L., et al., Biochem. Biophys. Res. Commun. 305(3):502-509 (2003).
Kimberly, W.T., et al., Proc. Natl. Acad. Sci. U.S.A. 100(11):6382-6387 (2003).
Edbauer, D., et al., Nat. Cell Biol. 5(5):486-488 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28996
NCBI Official Full Name
gamma-secretase subunit APH-1A isoform 1
NCBI Official Synonym Full Names
APH1A gamma secretase subunit
NCBI Official Symbol
APH1A
NCBI Official Synonym Symbols
APH-1; APH-1A; CGI-78; 6530402N02Rik
NCBI Protein Information
gamma-secretase subunit APH-1A
UniProt Protein Name
Gamma-secretase subunit APH-1A
UniProt Gene Name
APH1A
UniProt Synonym Gene Names
PSF; APH-1a
UniProt Entry Name
APH1A_HUMAN
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Product Notes
The APH1A aph1a (Catalog #AAA284484) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 77-109. The APH1 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's APH1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the APH1A aph1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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