FCM/FACS (Flow Cytometry)
(Blank control: A431. Primary Antibody (green line): Rabbit Anti-APH1a antibody (bs-4259R) Dilution: 1 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 ug /test.Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
Rabbit APH1a Polyclonal Antibody | anti-APH1a antibody
APH1a Polyclonal Antibody
Gene Names
APH1A; APH-1; APH-1A; CGI-78; 6530402N02Rik
Reactivity
Rat, Pig, Dog, Cow
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
APH1a, Antibody; APH1a Polyclonal Antibody; Gamma-secretase subunit APH-1A; APH-1a; Aph-1alpha; Presenilin-stabilization factor; APH1A; PSF; anti-APH1a antibody
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-APH1a antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Blank control: A431. Primary Antibody (green line): Rabbit Anti-APH1a antibody (bs-4259R) Dilution: 1 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 ug /test.Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Blank control: A431. Primary Antibody (green line): Rabbit Anti-APH1a antibody (bs-4259R) Dilution: 1 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 ug /test.Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
IF (Immunofluorescence)
(Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-APH1a Polyclonal Antibody, Unconjugated(bs-4259R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
IF (Immunofluorescence)
(Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-APH1a Polyclonal Antibody, Unconjugated(bs-4259R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
20,977 Da
NCBI Official Full Name
Anterior pharynx defective 1 homolog A (C. elegans)
NCBI Official Synonym Full Names
APH1A gamma secretase subunit
NCBI Official Symbol
APH1A
NCBI Official Synonym Symbols
APH-1; APH-1A; CGI-78; 6530402N02Rik
NCBI Protein Information
gamma-secretase subunit APH-1A; aph-1alpha; presenilin-stabilization factor; anterior pharynx defective 1 homolog A
UniProt Protein Name
Gamma-secretase subunit APH-1A
UniProt Gene Name
APH1A
UniProt Synonym Gene Names
PSF; APH-1a
UniProt Entry Name
APH1A_HUMAN
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Product Notes
The APH1a aph1a (Catalog #AAA291977) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APH1a Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's APH1a can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the APH1a aph1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APH1a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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