IF (Immunofluorescence)
(AAA328951 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328951 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit APH1a Polyclonal Antibody | anti-APH1A antibody
APH1a Antibody
Gene Names
APH1A; APH-1; APH-1A; CGI-78; 6530402N02Rik
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
APH1a, Antibody; APH1a Antibody; 6530402N02Rik; AL138795.3; Anterior Pharynx Defective 1; Anterior pharynx defective 1 homolog A; APH 1A; Aph 1alpha; APH-1a; Aph-1alpha; Aph1a; APH1A gamma secretase subunit; APH1A_HUMAN; CGI 78; CGI78; Gamma secretase subunit APH 1A; Gamma Secretase Subunit APH1a; Gamma-secretase subunit APH-1A; Likely ortholog of C. elegans anterior pharynx defective 1A; Presenilin Stabilization Factor; Presenilin-stabilization factor; PSF; UNQ579/PRO1141; anti-APH1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
APH1a Antibody detects endogenous levels of total APH1a.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
247
Applicable Applications for anti-APH1A antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
A synthesized peptide derived from human APH1a, corresponding to a region within C-terminal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328951 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328951 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA328951 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328951 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IHC (Immunohiostchemistry)
(AAA328951 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328951 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from K562, using APH1a Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from K562, using APH1a Antibody. The lane on the left was treated with blocking peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
28kDa
NCBI Official Full Name
Anterior pharynx defective 1 homolog A (C. elegans)
NCBI Official Synonym Full Names
aph-1 homolog A, gamma-secretase subunit
NCBI Official Symbol
APH1A
NCBI Official Synonym Symbols
APH-1; APH-1A; CGI-78; 6530402N02Rik
NCBI Protein Information
gamma-secretase subunit APH-1A
UniProt Protein Name
Gamma-secretase subunit APH-1A
UniProt Gene Name
APH1A
UniProt Synonym Gene Names
PSF; APH-1a
UniProt Entry Name
APH1A_HUMAN
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Product Notes
The APH1A aph1a (Catalog #AAA328951) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APH1a Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's APH1a can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the APH1A aph1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APH1a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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