WB (Western Blot)
(Detection of Ubiquitin by western blotting of Products from an Ubiquitination Assay and Purified Ubiquitin. Samples: A) Ubiquitination assay was performed as described in Zhang et al., 2004, Mol. Cell. Biol. 24(24):10941-10953. B) Purified ubiquitin applied in decreasing amounts from 5 to 0.1 ug. Antibody: Affinity purified rabbit anti-Ubiquitin antibody (Cat. No. AAA210885) used at 0.4 ug/ml for western blot. Detection: Alkaline phosphatase conjugated secondary antibody and development with NBT/BCIP for 10 minutes (A and B).)
Rabbit Ubiquitin Polyclonal Antibody | anti-UBA52 antibody
Rabbit anti-Ubiquitin Antibody, Affinity Purified
Reactivity
Human, Mouse, Bovine
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Ubiquitin, Antibody; Rabbit anti-Ubiquitin Antibody, Affinity Purified; ubiquitin carboxyl extension protein 52; CEP52; ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-52 amino acid fusion protein; L40; RPL40; HUBCEP52; Ubiquitin A-52 residue ribosomal protein fusion product 1; Ubiquitin-60S ribosomal protein L40; ubiquitin A-52 residue ribosomal protein fusion product 1; anti-UBA52 antibody
Host
Rabbit
Reactivity
Human, Mouse, Bovine
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-UBA52 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Predicted Reactivity
Rat, D. melanogaster, X. laevis, Chicken, Sheep, Dog, Horse, Rabbit, Pig, Cat, Gorilla, Chimpanzee
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of Ubiquitin by western blotting of Products from an Ubiquitination Assay and Purified Ubiquitin. Samples: A) Ubiquitination assay was performed as described in Zhang et al., 2004, Mol. Cell. Biol. 24(24):10941-10953. B) Purified ubiquitin applied in decreasing amounts from 5 to 0.1 ug. Antibody: Affinity purified rabbit anti-Ubiquitin antibody (Cat. No. AAA210885) used at 0.4 ug/ml for western blot. Detection: Alkaline phosphatase conjugated secondary antibody and development with NBT/BCIP for 10 minutes (A and B).)
WB (Western Blot)
(Detection of Ubiquitin by western blotting of Products from an Ubiquitination Assay and Purified Ubiquitin. Samples: A) Ubiquitination assay was performed as described in Zhang et al., 2004, Mol. Cell. Biol. 24(24):10941-10953. B) Purified ubiquitin applied in decreasing amounts from 5 to 0.1 ug. Antibody: Affinity purified rabbit anti-Ubiquitin antibody (Cat. No. AAA210885) used at 0.4 ug/ml for western blot. Detection: Alkaline phosphatase conjugated secondary antibody and development with NBT/BCIP for 10 minutes (A and B).)
IHC (Immunohistochemistry)
(Detection of human and mouse Ubiquitin by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-Ubiquitin (Cat. No. AAA210885 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human and mouse Ubiquitin by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-Ubiquitin (Cat. No. AAA210885 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-UBA52 antibody
Ubiquitin (Ub) is a small 76aa highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins provides an important means to regulate protein abundance and activity by tagging them for degradation. Ubiquitination is a highly regulated and reversible process that appears to be involved in the regulation of many cellular processes and a variety of clinical disorders.
Product Categories/Family for anti-UBA52 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The UBA52 uba52 (Catalog #AAA210885) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Ubiquitin Antibody, Affinity Purified reacts with Human, Mouse, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the UBA52 uba52 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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