WB (Western Blot)
(Western Blot of Peroxidase conjugated beta 2 Microglobulin primary antibody. Lane 1: beta 2 Microglobulin. Lane 2: None. Load: 50 ng per lane. None. Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 12 kDa, 12 kDa for Beta-2-Microglobulin..)
Rabbit beta 2 Microglobulin Polyclonal Antibody | anti-B2M antibody
beta 2 Microglobulin antibody (HRP)
Reactivity
Human beta-2-macrogloculin
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Beta 2 Microglobulin antibody (HRP) was purified by ion exchange chromatography followed by dialysis.
Synonyms
beta 2 Microglobulin, Antibody; beta 2 Microglobulin antibody (HRP); Polyclonal beta 2 Microglobulin; Anti-beta 2 Microglobulin; B2M; beta Microglobulin-2; beta Microglobulin 2; beta 2 microglobulin precursor; beta chain of mhc class 1 proteins; beta 2 Microglobulin; Hdcma22p; anti-B2M antibody
Host
Rabbit
Reactivity
Human beta-2-macrogloculin
Clonality
Polyclonal
Specificity
Human beta-2-macrogloculin
Purity/Purification
Beta 2 Microglobulin antibody (HRP) was purified by ion exchange chromatography followed by dialysis.
Form/Format
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA and 0.01% gentamycin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.
Sequence Length
119
Applicable Applications for anti-B2M antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Tag/Conjugate
HRP
Immunogen
Beta-2 microglobulin antibody (HRP) was raised in rabbit using beta-2-microglobulin from human urine as the immunogen.
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
WB (Western Blot)
(Western Blot of Peroxidase conjugated beta 2 Microglobulin primary antibody. Lane 1: beta 2 Microglobulin. Lane 2: None. Load: 50 ng per lane. None. Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 12 kDa, 12 kDa for Beta-2-Microglobulin..)
WB (Western Blot)
(Western Blot of Peroxidase conjugated beta 2 Microglobulin primary antibody. Lane 1: beta 2 Microglobulin. Lane 2: None. Load: 50 ng per lane. None. Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 12 kDa, 12 kDa for Beta-2-Microglobulin..)
IF (Immunofluorescence)
(Tissue: Human Embryonic Kidney cells (HEK 293 cells). Fixation: 2% paraformaldehyde for 15 minutes. beta 2 Microglobulin antibody at 1:200 for overnight at 4 deg C. Donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 488 fluorochrome at 1:1,000 for 45 min at RT. Localization: beta 2 microglobulin is mainly on the cell surface and occasionally cytoplasmic. Staining: beta 2 microglobulin as green fluorescent signal.)
IF (Immunofluorescence)
(Tissue: Human Embryonic Kidney cells (HEK 293 cells). Fixation: 2% paraformaldehyde for 15 minutes. beta 2 Microglobulin antibody at 1:200 for overnight at 4 deg C. Donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 488 fluorochrome at 1:1,000 for 45 min at RT. Localization: beta 2 microglobulin is mainly on the cell surface and occasionally cytoplasmic. Staining: beta 2 microglobulin as green fluorescent signal.)
IHC (Immunohistochemistry)
(Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Left panel: isotype control, Right panel:beta 2 Microglobulin antibody at 1 ug/ml for 20 min at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: beta 2 Microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Staining: beta 2 Microglobulin as brown with diaminobenzidine and with a hematoxylin purple counterstain.)
IHC (Immunohistochemistry)
(Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Left panel: isotype control, Right panel:beta 2 Microglobulin antibody at 1 ug/ml for 20 min at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: beta 2 Microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Staining: beta 2 Microglobulin as brown with diaminobenzidine and with a hematoxylin purple counterstain.)
Related Product Information for anti-B2M antibody
Rabbit polyclonal beta 2 Microglobulin antibody (HRP) conjugated
Product Categories/Family for anti-B2M antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
13,715 Da
NCBI Official Full Name
beta-2-microglobulin
NCBI Official Synonym Full Names
beta-2-microglobulin
NCBI Official Symbol
B2M
NCBI Protein Information
beta-2-microglobulin
UniProt Protein Name
Beta-2-microglobulin
UniProt Gene Name
B2M
UniProt Entry Name
B2MG_HUMAN
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Product Notes
The B2M b2m (Catalog #AAA75135) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The beta 2 Microglobulin antibody (HRP) reacts with Human beta-2-macrogloculin and may cross-react with other species as described in the data sheet. AAA Biotech's beta 2 Microglobulin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the B2M b2m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta 2 Microglobulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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