Rabbit anti-Human BRCA1 Polyclonal Antibody | anti-BRCA1 antibody

BRCA1 Antibody

Cat. Num. AAA29702

• Gene Names: BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: BRCA1; Polyclonal Antibody; BRCA1 Antibody; BRCAI; BRCC1; BROVCA1; IRIS; anti-BRCA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total BRCA1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1863

• Applicable Applications for anti-BRCA1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Peptide
• Immunogen Description: A synthetic peptide of human BRCA1.
• Target Name: BRCA1
• Preparation and Storage: Store at -20 degree C

IF (Immunofluorescence)

(Immunofluorescence analysis of A549 cell using BRCA1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded rat lung using BRCA1 antibody at dilution of 1:100 (400x lens).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded rat kidney using BRCA1 antibody at dilution of 1:100 (400x lens).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse lung using BRCA1 antibody at dilution of 1:100 (400x lens).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human esophageal cancer using BRCA1 antibody at dilution of 1:100 (400x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using BRCA1 antibody.)

Related Product Information for anti-BRCA1 antibody

The breast cancer susceptibility proteins BRCA1 and BRCA2 are frequently mutated in cases of hereditary breast and ovarian cancers and have roles in multiple processes related to DNA damage, repair, cell cycle progression, transcription, ubiquitination and apoptosis (1-4). BRCA2 has been shown to be required for localization of Rad51 to sites of double stranded breaks (DSBs) in DNA, and cells lacking BRCA1 and BRCA2 cannot repair DSBs through the Rad51-dependent process of homologous recombination (HR) (5). Numerous DNA-damage induced phosphorylation sites on BRCA1 have been identified, including serines 988, 1189, 1387, 1423, 1457, 1524 and 1542, and kinases activated in a cell cycle-dependent manner, including Aurora A and CDK2, can also phosphorylate BRCA1 at Ser308 and Ser1497, respectively (6-10). Cell cycle-dependent phosphorylation of BRCA2 at Ser3291 by CDKs has been proposed as a mechanism to switch off HR as cells progress beyond S-phase by blocking the carboxy-terminal Rad51 binding site (11).

Product Categories/Family for anti-BRCA1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 6552299
• NCBI GeneID: 672
• NCBI Accession #: NP_009225.1
• NCBI GenBank Nucleotide #: NM_007294.3
• UniProt Accession #: P38398; O15129; Q1RMC1; Q3LRJ0; Q3LRJ6; Q6IN79; Q7KYU9; E9PFZ0
• Molecular Weight: 202,342 Da
• NCBI Official Full Name: breast cancer type 1 susceptibility protein isoform 1
• NCBI Official Synonym Full Names: breast cancer 1, early onset
• NCBI Official Symbol: BRCA1
• NCBI Official Synonym Symbols: IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
• NCBI Protein Information: breast cancer type 1 susceptibility protein
• UniProt Protein Name: Breast cancer type 1 susceptibility protein
• UniProt Gene Name: BRCA1
• UniProt Synonym Gene Names: RNF53
• UniProt Entry Name: BRCA1_HUMAN