IHC (Immunohiostchemistry)
(Subnuclear distribution of cellular proteins. CHOBgl40 cells grown on coverslips were either directly or after treatment with 0.5% Triton X-100, incubated with antibodies against Brd4 (images 4 and 5). PI, propidium iodide staining of cellular DNA (images 6).WC, whole cells.)
Rabbit BRD4 Polyclonal Antibody | anti-BRD4 antibody
BRD4 Antibody (C-term)
Gene Names
BRD4; CAP; MCAP; HUNK1; HUNKI
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
BRD4, Antibody; BRD4 Antibody (C-term); Bromodomain-containing protein 4; Protein HUNK1; BRD4; HUNK1; anti-BRD4 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This BRD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1313~1342 amino acids from the C-terminal region of human BRD4.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
1313-1342
Sequence Length
722
Applicable Applications for anti-BRD4 antibody
IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Subnuclear distribution of cellular proteins. CHOBgl40 cells grown on coverslips were either directly or after treatment with 0.5% Triton X-100, incubated with antibodies against Brd4 (images 4 and 5). PI, propidium iodide staining of cellular DNA (images 6).WC, whole cells.)
IHC (Immunohiostchemistry)
(Subnuclear distribution of cellular proteins. CHOBgl40 cells grown on coverslips were either directly or after treatment with 0.5% Triton X-100, incubated with antibodies against Brd4 (images 4 and 5). PI, propidium iodide staining of cellular DNA (images 6).WC, whole cells.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-BRD4 antibody
BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. The gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people.
Product Categories/Family for anti-BRD4 antibody
References
Maruyama, T., et al., Mol. Cell. Biol. 22(18):6509-6520 (2002).
French, C.A., et al., Am. J. Pathol. 159(6):1987-1992 (2001).
Dey, A., et al., Mol. Cell. Biol. 20(17):6537-6549 (2000).
French, C.A., et al., Am. J. Pathol. 159(6):1987-1992 (2001).
Dey, A., et al., Mol. Cell. Biol. 20(17):6537-6549 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
152219
NCBI Official Full Name
bromodomain-containing protein 4 isoform short
NCBI Official Synonym Full Names
bromodomain containing 4
NCBI Official Symbol
BRD4
NCBI Official Synonym Symbols
CAP; MCAP; HUNK1; HUNKI
NCBI Protein Information
bromodomain-containing protein 4
UniProt Protein Name
Bromodomain-containing protein 4
UniProt Gene Name
BRD4
UniProt Synonym Gene Names
HUNK1
UniProt Entry Name
BRD4_HUMAN
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Product Notes
The BRD4 brd4 (Catalog #AAA285914) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1313-1342. The BRD4 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's BRD4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the BRD4 brd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRD4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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