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WB (Western Blot)
(Western blot analysis of CARD6 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). CARD6 (arrow) was detected using the purified Pab.)
Rabbit anti-Human CARD6 Polyclonal Antibody | anti-CARD6 antibody
CARD6 Antibody (Center)
Gene Names
CARD6; CINCIN1
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CARD6; Polyclonal Antibody; CARD6 Antibody (Center); Caspase recruitment domain-containing protein 6; anti-CARD6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CARD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human CARD6.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
1,037
Applicable Applications for anti-CARD6 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC~~1:25
WB~~1:1000
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of CARD6 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). CARD6 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of CARD6 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). CARD6 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(CARD6 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the CARD6 antibody detected the CARD6 protein (arrow).)
WB (Western Blot)
(CARD6 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the CARD6 antibody detected the CARD6 protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from A549, HepG2 cell line (from left to right), using CARD6 Antibody (Center). AAA28742 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from A549, HepG2 cell line (from left to right), using CARD6 Antibody (Center). AAA28742 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from WiDr cell line, using CARD6 Antibody (Center). AAA28742 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from WiDr cell line, using CARD6 Antibody (Center). AAA28742 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 20ug.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody (Center). AAA28742 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody (Center). AAA28742 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. colon section using CARD6 Antibody (Center). AAA28742 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. colon section using CARD6 Antibody (Center). AAA28742 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody (Center). AAA28742 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody (Center). AAA28742 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Related Product Information for anti-CARD6 antibody
CARD6 is a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB.
Product Categories/Family for anti-CARD6 antibody
References
Kim,S.S., et.al., Pathology 42 (1), 50-53 (2010)
Olsen,J.V., et.al., Cell 127 (3), 635-648 (2006)
Olsen,J.V., et.al., Cell 127 (3), 635-648 (2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
116468
NCBI Official Full Name
caspase recruitment domain-containing protein 6
NCBI Official Synonym Full Names
caspase recruitment domain family, member 6
NCBI Official Symbol
CARD6
NCBI Official Synonym Symbols
CINCIN1
NCBI Protein Information
caspase recruitment domain-containing protein 6
UniProt Protein Name
Caspase recruitment domain-containing protein 6
UniProt Gene Name
CARD6
UniProt Entry Name
CARD6_HUMAN
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Product Notes
The CARD6 card6 (Catalog #AAA28742) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CARD6 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CARD6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). IHC~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the CARD6 card6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARD6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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