IHC (Immunohistochemistry)
(AAA13655 Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.)
Goat CCKBR Polyclonal Antibody | anti-CCKBR antibody
Goat anti-CCKBR Antibody
Gene Names
CCKBR; GASR; CCK-B; CCK2R
Reactivity
Tested: Human, MouseExpected from sequence similarity: Human, Mouse, Rat, Cow
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
CCKBR, Antibody; Goat anti-CCKBR Antibody; CCKBR; cholecystokinin B receptor; HGNC: 1571; CCK-B; GASR; CCK2 receptor; cholecystokinin-B receptor/gastrin receptor; gastrin receptor; gastrin\cholecystokinin brain receptor; CCK2R; CCKBR antibody; cholecystokinin B receptor antibody; HGNC: 1571 antibody; CCK-B antibody; GASR antibody; CCK2 receptor antibody; cholecystokinin-B receptor/gastrin receptor antibody; gastrin receptor antibody; gastrin\cholecystokinin brain receptor antibody; CCK2R antibody; anti-CCKBR antibody
Host
Goat
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow
Expected from sequence similarity: Human, Mouse, Rat, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
FDGDSDSDSQSRVRNQ
Sequence Length
447
Applicable Applications for anti-CCKBR antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48-50kDa band observed in Mouse Pancreas lysates (calculated MW of 49.2kDa according to Mouse NP_031653.1) Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Pancreas. Recommended concentration: 4-6µg/ml.
Western blot: Approx 48-50kDa band observed in Mouse Pancreas lysates (calculated MW of 49.2kDa according to Mouse NP_031653.1) Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Pancreas. Recommended concentration: 4-6µg/ml.
Immunogen
Peptide with sequence C-FDGDSDSDSQSRVRNQ, from the internal region of the protein sequence according to NP_795344.1.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
IHC (Immunohistochemistry)
(AAA13655 Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.)
IHC (Immunohistochemistry)
(AAA13655 Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.)
IHC (Immunohistochemistry)
(AAA13655 (6µg/ml) staining of paraffin embedded Human Pancreas. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)
IHC (Immunohistochemistry)
(AAA13655 (6µg/ml) staining of paraffin embedded Human Pancreas. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
gastrin/cholecystokinin type B receptor isoform 1
NCBI Official Synonym Full Names
cholecystokinin B receptor
NCBI Official Symbol
CCKBR
NCBI Official Synonym Symbols
GASR; CCK-B; CCK2R
NCBI Protein Information
gastrin/cholecystokinin type B receptor
UniProt Protein Name
Gastrin/cholecystokinin type B receptor
UniProt Gene Name
CCKBR
UniProt Synonym Gene Names
CCKRB; CCK-B receptor; CCK-BR; CCK2-R
UniProt Entry Name
GASR_HUMAN
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Product Notes
The CCKBR cckbr (Catalog #AAA13655) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CCKBR Antibody reacts with Tested: Human, Mouse Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CCKBR can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48-50kDa band observed in Mouse Pancreas lysates (calculated MW of 49.2kDa according to Mouse NP_031653.1) Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Pancreas. Recommended concentration: 4-6µg/ml. Researchers should empirically determine the suitability of the CCKBR cckbr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: FDGDSDSDSQ SRVRNQ. It is sometimes possible for the material contained within the vial of "CCKBR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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