IHC (Immunohistochemistry)
(Immunohistochemistry in formalin-fixed and paraffin-embedded human hepatocarcinoma using AAA24067, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of AAA24067 for immunohistochemistry.)
Rabbit anti-Human CD163 Polyclonal Antibody | anti-CD163 antibody
CD163, NT (CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163, Soluble CD163)
Gene Names
CD163; M130; MM130
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified by ammonium sulfate precipitation.
Synonyms
CD163, Antibody; CD163, NT (CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163, Soluble CD163); Anti -CD163, NT (CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163, Soluble CD163); anti-CD163 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CD163
Purity/Purification
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Concentration
2.04 mg/mL (varies by lot)
Applicable Applications for anti-CD163 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Other applications have not been tested.
Application Notes
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:10-1:50
Optimal dilutions to be determined by the researcher.
Immunohistochemistry (FFPE): 1:10-1:50
Optimal dilutions to be determined by the researcher.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragement of residues within amino acids 245-274 in the N-terminal region of human CD163.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
IHC (Immunohistochemistry)
(Immunohistochemistry in formalin-fixed and paraffin-embedded human hepatocarcinoma using AAA24067, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of AAA24067 for immunohistochemistry.)
IHC (Immunohistochemistry)
(Immunohistochemistry in formalin-fixed and paraffin-embedded human hepatocarcinoma using AAA24067, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of AAA24067 for immunohistochemistry.)
WB (Western Blot)
(Western Blot analysis in K562 cell line lysates (35ug/lane) using AAA24067. CD163 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western Blot analysis in K562 cell line lysates (35ug/lane) using AAA24067. CD163 (arrow) was detected using the purified Pab.)
Related Product Information for anti-CD163 antibody
CD163 is an acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. The protein may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. It binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. And it exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. It also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.
Product Categories/Family for anti-CD163 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
CD163
NCBI Official Synonym Full Names
CD163 molecule
NCBI Official Symbol
CD163
NCBI Official Synonym Symbols
M130; MM130
NCBI Protein Information
scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen
UniProt Protein Name
Scavenger receptor cysteine-rich type 1 protein M130
UniProt Gene Name
CD163
UniProt Synonym Gene Names
M130; sCD163
UniProt Entry Name
C163A_HUMAN
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Product Notes
The CD163 cd163 (Catalog #AAA24067) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD163, NT (CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163, Soluble CD163) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD163 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Other applications have not been tested. Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the CD163 cd163 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD163, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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