Rabbit anti-Human CD19 Polyclonal Antibody | anti-CD19 antibody

CD19 Antibody (N-term)

Cat. Num. AAA28685

• Gene Names: CD19; B4; CVID3
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry, ELISA
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: CD19, Antibody; CD19 Antibody (N-term); B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19; anti-CD19 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CD19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-172 amino acids from the N-terminal region of human CD19.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 143-172

• Applicable Applications for anti-CD19 antibody: Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)
• Application Notes: WB~~1:500; IF~~1:10~50; FC~~1:10~50; IHC-P~~1:10~50
• Function: Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B- cell responses to antigens (PubMed:2463100, PubMed:1373518, PubMed:16672701). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:9382888, PubMed:9317126, PubMed:12387743, PubMed:16672701). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:2463100, PubMed:1373518). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:9317126, PubMed:12387743, PubMed:16672701).
• Cellular Location: Cell membrane; Single- pass type I membrane protein. Membrane raft {ECO:0000250|UniProtKB:P25918}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P25918}
• Tissue Location: Detected on marginal zone and germinal center B cells in lymph nodes (PubMed:2463100). Detected on blood B cells (at protein level) (PubMed:2463100, PubMed:16672701)
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistchemistry)

(Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

FCM (Flow Cytometry)

(Flow cytometric analysis of CEM cells using CD19 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence)

(Immunofluorescence analysis of CD19 Antibody (N-term) with paraffin-embedded human lymph tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)

WB (Western Blot)

(Western blot analysis of CD19 (arrow) using rabbit polyclonal CD19 Antibody (N-term) AAA28685. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (Lane 2) (Origene Technologies).)

WB (Western Blot)

(CD19 Antibody (N-term) AAA28685 western blot analysis in Raji cell line lysates (35ug/lane).This demonstrates the CD19 antibody detected the CD19 protein (arrow).)

WB (Western Blot)

(Western blot analysis of lysate from Ramos cell line, using CD19Antibody (N-term). AAA28685 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

Related Product Information for anti-CD19 antibody

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Product Categories/Family for anti-CD19 antibody

Crown Antibodies; Cancer; Immunology; Signal Transduction

References

Deaglio,S., Blood 109 (12), 5390-5398 (2007)
Bradbury,L.E., J. Immunol. 149 (9), 2841-2850 (1992)
Kozmik,Z., Mol. Cell. Biol. 12 (6), 2662-2672 (1992)

NCBI and Uniprot Product Information

• NCBI GI #: 296010921
• NCBI GeneID: 930
• NCBI Accession #: NP_001171569.1; NP_001761.3
• NCBI GenBank Nucleotide #: NM_001178098.1
• UniProt Accession #: P15391; Q96S68; Q9BRD6; A0N0P9; F5H635
• NCBI Official Full Name: B-lymphocyte antigen CD19 isoform 1
• NCBI Official Synonym Full Names: CD19 molecule
• NCBI Official Symbol: CD19
• NCBI Official Synonym Symbols: B4; CVID3
• NCBI Protein Information: B-lymphocyte antigen CD19
• UniProt Protein Name: B-lymphocyte antigen CD19
• UniProt Gene Name: CD19
• UniProt Entry Name: CD19_HUMAN