WB (Western Blot)
(Western blot analysis of various lysates, using CD47 antibody (AAA282419) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.)
Rabbit anti-Mouse, Rat CD47 Polyclonal Antibody | anti-CD47 antibody
CD47 Rabbit pAb
Gene Names
Cd47; IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
CD47, Antibody; CD47 Rabbit pAb; IAP; Itgp; 9130415E20Rik; B430305P08Rik; anti-CD47 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Applicable Applications for anti-CD47 antibody
WB (Western Blot)
Positive Samples
Mouse thymus, Rat spleen, Rat lung
Immunogen
Recombinant protein of mouse CD47.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Ship: Ice Pack
Ship: Ice Pack
WB (Western Blot)
(Western blot analysis of various lysates, using CD47 antibody (AAA282419) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of various lysates, using CD47 antibody (AAA282419) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of Mouse thymus, using CD47 antibody (AAA282419) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of Mouse thymus, using CD47 antibody (AAA282419) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.)
Related Product Information for anti-CD47 antibody
Predicted to enable protein binding activity involved in heterotypic cell-cell adhesion and thrombospondin receptor activity. Involved in regulation of nitric oxide biosynthetic process. Acts upstream of or within several processes, including monocyte aggregation; opsonization; and positive regulation of phagocytosis. Located in extracellular exosome. Is integral component of plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; hemolymphoid system gland; and liver and biliary system. Orthologous to human CD47 (CD47 molecule).
Product Categories/Family for anti-CD47 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,098 Da
NCBI Official Full Name
leukocyte surface antigen CD47
NCBI Official Synonym Full Names
CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
NCBI Official Symbol
Cd47
NCBI Official Synonym Symbols
IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
NCBI Protein Information
leukocyte surface antigen CD47; Rh-related antigen; integrin-associated protein
UniProt Protein Name
Leukocyte surface antigen CD47
UniProt Gene Name
Cd47
UniProt Synonym Gene Names
IAP
UniProt Entry Name
CD47_MOUSE
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Product Notes
The CD47 cd47 (Catalog #AAA282419) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD47 Rabbit pAb reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD47 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the CD47 cd47 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD47, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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