WB (Western Blot)
(Anti-CD82 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:32 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human CD82 Polyclonal Antibody | anti-CD82 antibody
Anti-CD82 Antibody, Rabbit Polyclonal
Gene Names
CD82; R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
CD82, Antibody; Anti-CD82 Antibody, Rabbit Polyclonal; CD82/KAI-1 Antibody, Rabbit PAb, Antigen Affinity Purified; CD82 molecule; Anti-4F9 Antibody; Anti-C33 Antibody; Anti-GR15 Antibody; Anti-IA4 Antibody; Anti-KAI-1 Antibody; Anti-KAI1 Antibody; Anti-R2 Antibody; Anti-SAR2 Antibody; Anti-ST6 Antibody; Anti-TSPAN27 Antibody; anti-CD82 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CD82/KAI-1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD82 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human CD82/KAI-1 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human CD82/KAI-1 (rh CD82/KAI-1). CD82/KAI-1 specific IgG was purified by Human CD82/KAI-1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-CD82 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:32 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-CD82 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:32 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(CD82 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell LysateLane B:0.5 mg U87MG Whole Cell Lysate2 uL anti-CD82 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD82 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 36 kDa)
IP (Immunoprecipitation)
(CD82 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell LysateLane B:0.5 mg U87MG Whole Cell Lysate2 uL anti-CD82 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD82 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 36 kDa)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD82 in human endometrium with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of epithelium in uterine-gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD82 in human endometrium with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of epithelium in uterine-gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Related Product Information for anti-CD82 antibody
CD82, also known as KAI-1, structurally belongs to tetraspanin family while categorised as metastasis suppressor gene on functional grounds. KAI1/CD82 is localized on cell membrane and form interactions with other tetraspanins, integrins and chemokines which are respectively responsible for cell migration, adhesion and signalling. Downregulation of CD82 expression is associated with the advanced stages of many human cancers and correlates with the acquisition of metastatic potential. Recent studies suggest that complex mechanisms underlie CD82 loss of function, including altered transcriptional regulation, splice variant production and post-translational protein modifications, and indicate a central role for CD82 in controlling metastasis as a 'molecular facilitator'. The loss of KAI1/CD82 expression in invasive and metastatic cancers is due to a complex, epigenetic mechanism that probably involves transcription factors such as NFkappaB, p53, and beta-catenin. A loss of KAI1 expression is also associated with the advanced stages of many human malignancies and results in the acquisition of invasive and metastatic capabilities by tumour cells. Thus, KAI1/CD82 is regarded as a wide-spectrum tumor metastasis suppressor.
References
Malik FA, et al. (2009) KAI-1/CD82, the molecule and clinical implication in cancer and cancer metastasis. Histol Histopathol. 24(4): 519-30.Liu WM, et al. (2006) KAI1/CD82, a tumor metastasis suppressor. Cancer Lett. 240(2): 183-94.Tonoli H, et al. (2006) CD82 metastasis suppressor gene: a potential target for new therapeutics? Trends Mol Med. 11(12): 563-70.Jackson P, et al. (2005) KAI1 tetraspanin and metastasis suppressor. Int J Biochem Cell Biol. 37(3): 530-4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,818 Da
NCBI Official Full Name
CD82 antigen isoform 1
NCBI Official Synonym Full Names
CD82 molecule
NCBI Official Symbol
CD82
NCBI Official Synonym Symbols
R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
NCBI Protein Information
CD82 antigen; tspan-27; C33 antigen; tetraspanin-27; inducible membrane protein R2; metastasis suppressor Kangai-1; kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4))
UniProt Protein Name
CD82 antigen
UniProt Gene Name
CD82
UniProt Synonym Gene Names
KAI1; SAR2; ST6; TSPAN27; Tspan-27
UniProt Entry Name
CD82_HUMAN
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Product Notes
The CD82 cd82 (Catalog #AAA255555) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD82 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD82 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD82 cd82 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD82, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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