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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells using CD82 (ST6) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse CD82 (ST6) Polyclonal Antibody | anti-CD82 antibody
CD82 (ST6) Antibody (C-term)
Gene Names
CD82; R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CD82 (ST6), Antibody; CD82 (ST6) Antibody (C-term); CD82 antigen; C33 antigen; IA4; Inducible membrane protein R2; Metastasis suppressor Kangai-1; Suppressor of tumorigenicity 6 protein; Tetraspanin-27; Tspan-27; CD82; KAI1; SAR2; ST6; TSPAN27; anti-CD82 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This CD82 (ST6) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-267 amino acids from the C-terminal region of human CD82 (ST6).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
239-267
Sequence Length
242
Applicable Applications for anti-CD82 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
Cellular Location
Cell membrane; Multi-pass membrane protein
!Tissue Location
Lymphoid specific.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells using CD82 (ST6) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells using CD82 (ST6) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human lymph with CD82 (ST6) Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human lymph with CD82 (ST6) Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Anti-ST6 Antibody (C253) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-ST6 Antibody (C253) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-ST6 Antibody (C253) at 1:1000 dilutionLane 1: Jurkat whole cell lysateLane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-ST6 Antibody (C253) at 1:1000 dilutionLane 1: Jurkat whole cell lysateLane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Western blot analysis of ST6 (arrow) using ST6 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD82 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of ST6 (arrow) using ST6 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD82 gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-CD82 antibody
The ST6 metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients.
Product Categories/Family for anti-CD82 antibody
References
Zhang, X.A., et al., J. Biol. Chem. 278(29):27319-27328 (2003).
Zhang, X.A., et al., Cancer Res. 63(10):2665-2674 (2003).
Yang, J., et al., Ai Zheng 22(5):533-536 (2003).
Sauer, G., et al., Oncol. Rep. 10(2):405-410 (2003).
Ito, Y., et al., Pathol Res Pract 199(2):79-83 (2003).
Zhang, X.A., et al., Cancer Res. 63(10):2665-2674 (2003).
Yang, J., et al., Ai Zheng 22(5):533-536 (2003).
Sauer, G., et al., Oncol. Rep. 10(2):405-410 (2003).
Ito, Y., et al., Pathol Res Pract 199(2):79-83 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29626
NCBI Official Full Name
CD82 antigen isoform 2
NCBI Official Synonym Full Names
CD82 molecule
NCBI Official Symbol
CD82
NCBI Official Synonym Symbols
R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
NCBI Protein Information
CD82 antigen
UniProt Protein Name
CD82 antigen
UniProt Gene Name
CD82
UniProt Synonym Gene Names
KAI1; SAR2; ST6; TSPAN27; Tspan-27
UniProt Entry Name
CD82_HUMAN
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Product Notes
The CD82 cd82 (Catalog #AAA285757) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 239-267. The CD82 (ST6) Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD82 (ST6) can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD82 cd82 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD82 (ST6), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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