Rabbit KAI1 Polyclonal Antibody | anti-CD82 antibody

KAI1 Antibody

Cat. Num. AAA39233

• Gene Names: CD82; R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: KAI1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: KAI1, Antibody; KAI1 Antibody; KAI1; R2; 4F9; C33; IA4; ST6; GR15; SAR2; TSPAN27; CD82 antigen; C33 antigen; Tspan-27; CD82 molecule; anti-CD82 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: KAI1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 267

• Applicable Applications for anti-CD82 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: KAI1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human KAI1.
• Buffer: KAI1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: KAI1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of KAI1 in Human Colon cells with KAI1 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of KAI1 in human colon tissue with KAI1 antibody at 2.5 μg/mL.)

WB (Western Blot)

(Western blot analysis of KAI1 in A549 cell lysate with KAI1 antibody at (A) 0.5 and (B) 1 μg/mL.)

Related Product Information for anti-CD82 antibody

KAI1 Antibody: KAI1 was initially identified from a T-cell activation study as a four-transmembrane protein that plays an accessory role in T-cell activation, and was later determined to act as a cancer metastasis suppressor gene. This protein is ubiquitously expressed at moderate to high levels in most tissues, but its expression is downregulated during tumor progression. The loss of KAI1 and p53 is associated with poor survival for prostate and other cancer patients. Recently, KAI1 was found to interact with DARC, the Duffy antigen for chemokines using a yeast two hybrid screen. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least two isoforms of KAI1 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 4504813
• NCBI GeneID: 3732
• NCBI Accession #: NP_002222
• NCBI GenBank Nucleotide #: NM_002231.3
• UniProt Accession #: P27701; Q7Z2D4; Q7Z5N2; D3DQN6; E9PC70
• Molecular Weight: 26,818 Da
• NCBI Official Full Name: CD82 antigen isoform 1
• NCBI Official Synonym Full Names: CD82 molecule
• NCBI Official Symbol: CD82
• NCBI Official Synonym Symbols: R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
• NCBI Protein Information: CD82 antigen; tspan-27; C33 antigen; tetraspanin-27; inducible membrane protein R2; metastasis suppressor Kangai-1; kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4))
• UniProt Protein Name: CD82 antigen
• UniProt Gene Name: CD82
• UniProt Synonym Gene Names: KAI1; SAR2; ST6; TSPAN27; Tspan-27
• UniProt Entry Name: CD82_HUMAN