WB (Western Blot)
(Anti-SLC3A2 rabbit polyclonal antibody at 1:500 dilution.Lane A: K562 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:68 kDaObserved band size:68 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Mouse CD98 Polyclonal Antibody | anti-SLC3A2 antibody
Anti-CD98 Antibody, Rabbit Polyclonal
Gene Names
Slc3a2; 4F2; Cd98; Ly10; Mdu1; 4F2HC; Ly-10; NACAE; Ly-m10; Mgp-2hc; AI314110
Reactivity
Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
CD98, Antibody; Anti-CD98 Antibody, Rabbit Polyclonal; SLC3A2/CD98 Antibody, Rabbit PAb, Antigen Affinity Purified; solute carrier family 3 (amino acid transporter heavy chain), member 2; Anti-4F2 Antibody; Anti-4F2HC Antibody; Anti-AI314110 Antibody; Anti-Cd98 Antibody; Anti-Ly-10 Antibody; Anti-Ly-m10 Antibody; Anti-Ly10 Antibody; Anti-Mdu1 Antibody; Anti-Mgp-2hc Antibody; Anti-NACAE Antibody; anti-SLC3A2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse SLC3A2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SLC3A2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Mouse SLC3A2 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse SLC3A2 (rM SLC3A2). SLC3A2 specific IgG was purified by Mouse SLC3A2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-SLC3A2 rabbit polyclonal antibody at 1:500 dilution.Lane A: K562 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:68 kDaObserved band size:68 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-SLC3A2 rabbit polyclonal antibody at 1:500 dilution.Lane A: K562 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:68 kDaObserved band size:68 kDa(We are unsure as to the identity of these extra bands.))
IHC (Immunohistochemistry)
(Immunochemical staining of mouse SLC3A2 in mouse kidney with rabbit polyclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nephric tubule. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
IHC (Immunohistochemistry)
(Immunochemical staining of mouse SLC3A2 in mouse kidney with rabbit polyclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nephric tubule. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Related Product Information for anti-SLC3A2 antibody
4F2 cell-surface antigen heavy chain, also known as 4F2 heavy chain antigen, Lymphocyte activation antigen 4F2 large subunit, CD98, SLC3A2 and MDU1, is a single-pass type I I membrane protein that belongs to the SLC3A transporter family. SLC3A2/MDU1 is expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta is significantly stronger at full-term than at the mid-trimester stage. SLC3A2/MDU1 is expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. It is expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. SLC3A2/MDU1 is also expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco2-BBE. SLC3A2/MDU1 is required for the function of light chain amino-acid transporters. It is involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. SLC3A2/MDU1 is involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7, SLC3A2/MDU1 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. SLC3A2/MDU1 plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. It is required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, SLC3A2/MDU1 is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta.
References
Torrents D. et al., 1998, J Biol Chem. 273: 32437-45. Pfeiffer R. et al., 1999, EMBO J. 18: 49-57. Broeer A. et al., 2000, Biochem J. 349: 787-95. Yanagida O. et al., 2001, Biochim Biophys Acta. 1514: 291-302. Broeer A. et al., 2001, Biochem J. 355: 725-31. Fort J. et al., 2007, J Biol Chem. 282: 31444-52. Mayya V. et al., 2009, Sci Signal. 2: RA46-RA46.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,240 Da
NCBI Official Full Name
4F2 cell-surface antigen heavy chain isoform a
NCBI Official Synonym Full Names
solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
NCBI Official Symbol
Slc3a2
NCBI Official Synonym Symbols
4F2; Cd98; Ly10; Mdu1; 4F2HC; Ly-10; NACAE; Ly-m10; Mgp-2hc; AI314110
NCBI Protein Information
4F2 cell-surface antigen heavy chain; CD98 antigen; CD98 heavy chain; antigen identified by monoclonal antibodies 4F2; solute carrier family 3 member 2; type II transmembrane protein
UniProt Protein Name
4F2 cell-surface antigen heavy chain
UniProt Gene Name
Slc3a2
UniProt Synonym Gene Names
Mdu1; 4F2hc
UniProt Entry Name
4F2_MOUSE
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Product Notes
The SLC3A2 slc3a2 (Catalog #AAA257231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD98 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD98 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SLC3A2 slc3a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD98, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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