IP (Immunoprecipitation)
(CDC42BPB was immunoprecipitated using:Lane A:0.5 mg U251MG Whole Cell Lysate4 uL anti-CDC42BPB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CDC42BPB rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 194 kDaObserved band size :194 kDa)
Rabbit anti-Human CDC42BPB Polyclonal Antibody | anti-CDC42BPB antibody
Anti-CDC42BPB Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
CDC42BPB, Antibody; Anti-CDC42BPB Antibody, Rabbit Polyclonal; CDC42BPB Antibody, Rabbit PAb, Antigen Affinity Purified; CDC42 binding protein kinase beta (DMPK-like); Anti-MRCKB Antibody; anti-CDC42BPB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CDC42BPB
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-CDC42BPB antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
E Coli-derived Human CDC42BPB fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human CDC42BPB fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(CDC42BPB was immunoprecipitated using:Lane A:0.5 mg U251MG Whole Cell Lysate4 uL anti-CDC42BPB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CDC42BPB rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 194 kDaObserved band size :194 kDa)
IP (Immunoprecipitation)
(CDC42BPB was immunoprecipitated using:Lane A:0.5 mg U251MG Whole Cell Lysate4 uL anti-CDC42BPB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CDC42BPB rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 194 kDaObserved band size :194 kDa)
WB (Western Blot)
(Anti-CDC42BPB rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: U 251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:194 kDaObserved band size: kDa)
WB (Western Blot)
(Anti-CDC42BPB rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: U 251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:194 kDaObserved band size: kDa)
Related Product Information for anti-CDC42BPB antibody
CDC42BPB is a member of the serine/threonine protein kinase family that contains a Cdc42/Rsc-binding p21 binding domain similar to that of PAK kinase. The kinase domain of this protein is related to the myotonic dystrophy kinase related ROK and this kinase may have functions in downstream regulating of Cdc42 in cytoskeletal recognization. It has been reported that the CDC42BPB protein take part in regulating numerous cellular functions by binding to members of a serine/threonine protein kinase subfamily. These functions include the remodeling of the cell cytoskeleton that is a feature of cell growth and differentiation.
References
Moncrieff CL, et al. (1999) Cloning and Chromosomal Localization of Human Cdc42-Binding Protein Kinase beta.Genomics. 57 (2): 297-300.Boyd Y, et al. (2001) Orthologs of seven genes (AKT1, CDC42BPB, DIO3, EIF5, JAG2, KLC, NDUFB1) from human Chromosome 14q32 map to distal chicken Chromosome 5. Mammalian Genome. 13 (2): 120-2.
Similar Products
Product Notes
The CDC42BPB (Catalog #AAA256534) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CDC42BPB Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDC42BPB can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CDC42BPB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC42BPB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.