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IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA18859 at dilution of 1:100)
Rabbit anti-Human Cellular tumor antigen p53 Polyclonal Antibody | anti-TP53 antibody
Rabbit anti-human Cellular tumor antigen p53 polyclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Synonyms
Cellular tumor antigen p53; Polyclonal Antibody; Rabbit anti-human Cellular tumor antigen p53 polyclonal Antibody; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53; anti-TP53 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation Purified
Sequence Length
393
Applicable Applications for anti-TP53 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: IHC:1:20-1:200
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human Cellular tumor antigen p53 protein(2-393aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-1314 / sc-1311-R / sc-6243
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA18859 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA18859 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA18859 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA18859 at dilution of 1:100)
WB (Western Blot)
(Western BlotPositive WB detected in: Mouse brain tissueAll lanes: TP53 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44,38,39,40,34,35,30,24,25 KDaObserved band size: 44 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Mouse brain tissueAll lanes: TP53 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44,38,39,40,34,35,30,24,25 KDaObserved band size: 44 KDa)
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation Hela(1.2^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-p53 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the P21 promoter.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation Hela(1.2^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-p53 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the P21 promoter.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta using AAA18859 at dilution 1:100 Chromatin Immunoprecipitation)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta using AAA18859 at dilution 1:100 Chromatin Immunoprecipitation)
WB (Western Blot)
(Western BlotPositive WB detected in:HEK293 whole cell lysate,NIH/3T3 whole cell lysate,A549 whole cell lysate,MCF-7 whole cell lysate,Mouse kidney tissueAll lanes: p53 antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44,38,39,40,34,35,30,24,25 kDaObserved band size: 44 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in:HEK293 whole cell lysate,NIH/3T3 whole cell lysate,A549 whole cell lysate,MCF-7 whole cell lysate,Mouse kidney tissueAll lanes: p53 antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44,38,39,40,34,35,30,24,25 kDaObserved band size: 44 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: mouse kidneyAll lanes: p53 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44,38,39,40,34,35,30,24,25 kDaObserved band size: 44 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: mouse kidneyAll lanes: p53 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44,38,39,40,34,35,30,24,25 kDaObserved band size: 44 kDa)
WB (Western Blot)
(Western blotAll lanes: Cellular tumor antigen p53 antibody at 2ug/mlLane 1:A431 whole cell lysateLane 2:293T whole cell lysateLane 3:MCF-7 whole cell lysateLane 4:MM231 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 44,38,39,40,34,35,30,24,25 kDaObserved band size: 44 kDa)
WB (Western Blot)
(Western blotAll lanes: Cellular tumor antigen p53 antibody at 2ug/mlLane 1:A431 whole cell lysateLane 2:293T whole cell lysateLane 3:MCF-7 whole cell lysateLane 4:MM231 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 44,38,39,40,34,35,30,24,25 kDaObserved band size: 44 kDa)
Related Product Information for anti-TP53 antibody
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediatedapoptosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,401 Da
NCBI Official Full Name
cellular tumor antigen p53 isoform a
NCBI Official Synonym Full Names
tumor protein p53
NCBI Official Symbol
TP53
NCBI Official Synonym Symbols
P53; BCC7; LFS1; TRP53
NCBI Protein Information
cellular tumor antigen p53
UniProt Protein Name
Cellular tumor antigen p53
UniProt Gene Name
TP53
UniProt Synonym Gene Names
P53
UniProt Entry Name
P53_HUMAN
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Product Notes
The TP53 tp53 (Catalog #AAA18859) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Cellular tumor antigen p53 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cellular tumor antigen p53 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: IHC:1:20-1:200. Researchers should empirically determine the suitability of the TP53 tp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cellular tumor antigen p53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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