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WB (Western Blot)
(Western Blot analysis of MCF7, K562 cells using Di-Methyl-p53 (K370) Polyclonal Antibody. Secondary antibody)
Rabbit p53 Polyclonal Antibody | anti-p53 antibody
p53 (Di Methyl Lys370) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Rat, Mouse
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
p53; Polyclonal Antibody; p53 (Di Methyl Lys370) Antibody; Cellular tumor antigen p53; TP53; P53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; anti-p53 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Di-Methyl-p53 (K370) Polyclonal Antibody detects endogenous levels of p53 protein only when di-methylated at K370.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-p53 antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IF: 1:50-200
WB: 1:500-1:2000
IHC: 1:100-300
ELISA: 1:20000
WB: 1:500-1:2000
IHC: 1:100-300
ELISA: 1:20000
Immunogen
Synthesized peptide derived from human p53 around the di-methylation site of K370.
Modification
Methyl Specific
Preparation and Storage
Store at -20 degree C/1 year
WB (Western Blot)
(Western Blot analysis of MCF7, K562 cells using Di-Methyl-p53 (K370) Polyclonal Antibody. Secondary antibody)
WB (Western Blot)
(Western Blot analysis of MCF7, K562 cells using Di-Methyl-p53 (K370) Polyclonal Antibody. Secondary antibody)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
IF (Immunofluorescence)
(Immunofluorescence analysis of human-liver-cancer tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of human-liver-cancer tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of human-kidney tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of human-kidney tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of human-breast-cancer tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of human-breast-cancer tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
Related Product Information for anti-p53 antibody
tumor protein p53(TP53) Homo sapiens This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs; 12032546, 20937277). [provided by RefSeq, Feb 2013]
Product Categories/Family for anti-p53 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,653 Da
NCBI Official Full Name
cellular tumor antigen p53 isoform a
NCBI Official Synonym Full Names
tumor protein p53
NCBI Official Symbol
TP53
NCBI Official Synonym Symbols
P53; BCC7; LFS1; TRP53
NCBI Protein Information
cellular tumor antigen p53; antigen NY-CO-13; phosphoprotein p53; p53 tumor suppressor; transformation-related protein 53
UniProt Protein Name
Cellular tumor antigen p53
UniProt Gene Name
TP53
UniProt Synonym Gene Names
P53
UniProt Entry Name
P53_HUMAN
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Product Notes
The p53 tp53 (Catalog #AAA30791) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p53 (Di Methyl Lys370) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p53 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). IF: 1:50-200 WB: 1:500-1:2000 IHC: 1:100-300 ELISA: 1:20000. Researchers should empirically determine the suitability of the p53 tp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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