IHC (Immunohiostchemistry)
(CHAT Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit CHAT Polyclonal Antibody | anti-CHAT antibody
CHAT Rabbit anti-Human Polyclonal (aa517-732) Antibody
Gene Names
CHAT; CMS6; CMS1A; CMS1A2; CHOACTASE
Reactivity
Mouse, Rat, Human
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
CHAT, Antibody; CHAT Rabbit anti-Human Polyclonal (aa517-732) Antibody; PathPlus CHAT Antibody (aa517-732); CHAT; Choline acetylase; Choline acetyltransferase; Choline O-acetyltransferase; CMS1A2; CHOACTASE; CMS1A; anti-CHAT antibody
Host
Rabbit
Reactivity
Mouse, Rat, Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ChAT.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
PBS, pH7.4, 0.05% Proclin-300, 50% glycerol
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-CHAT antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Target
Human CHAT
Immunogen
Recombinant ChAT (Asp517-Pro732)
Conjugation
Unconjugated
Epitope
aa517-732
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
IHC (Immunohiostchemistry)
(CHAT Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohiostchemistry)
(CHAT Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(CHAT Antibody-Western blot of CHAT antibody.)
WB (Western Blot)
(CHAT Antibody-Western blot of CHAT antibody.)
Related Product Information for anti-CHAT antibody
CHAT antibody is an unconjugated rabbit polyclonal antibody to CHAT (aa517-732) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
CHAT (choline acetyltransferase, CAT, CHOACTASE) is an enzyme that catalyzes the biosynthesis of the neurotransmitter acetylcholine. CHAT has important functions in the cholinergic system and is expressed by (and may be used to identify) cholinergic neurons, whose neurodegeneration is a typical component of Alzheimer's disease. CHAT variants and change-of-function mutations are correlated with Alzheimer's disease, mild cognitive impairment, and congenital myasthenic syndrome with episodic apnea. In immunohistochemistry, CHAT has cytoplasmic positivity in cholinergic nerve endings in the brain, and is also found in specific cell populations in the gastrointestinal tract and in placental stroma.
CHAT (choline acetyltransferase, CAT, CHOACTASE) is an enzyme that catalyzes the biosynthesis of the neurotransmitter acetylcholine. CHAT has important functions in the cholinergic system and is expressed by (and may be used to identify) cholinergic neurons, whose neurodegeneration is a typical component of Alzheimer's disease. CHAT variants and change-of-function mutations are correlated with Alzheimer's disease, mild cognitive impairment, and congenital myasthenic syndrome with episodic apnea. In immunohistochemistry, CHAT has cytoplasmic positivity in cholinergic nerve endings in the brain, and is also found in specific cell populations in the gastrointestinal tract and in placental stroma.
References
Curr Neuropharmacol. 2016 Jan; 14(1): 101 -115, PMID: 26813123; Dement Geriatr Cogn Disord. 2008;26(1):9-14, PMID: 18562794; J Neuropathol Exp Neurol. 57 (1): 63 -75, PMID: 9600198;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70,394 Da
NCBI Official Full Name
choline O-acetyltransferase isoform 2
NCBI Official Synonym Full Names
choline O-acetyltransferase
NCBI Official Symbol
CHAT
NCBI Official Synonym Symbols
CMS6; CMS1A; CMS1A2; CHOACTASE
NCBI Protein Information
choline O-acetyltransferase
UniProt Protein Name
Choline O-acetyltransferase
UniProt Gene Name
CHAT
UniProt Synonym Gene Names
CHOACTase; ChAT; Choline acetylase
UniProt Entry Name
CLAT_HUMAN
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Product Notes
The CHAT chat (Catalog #AAA162802) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHAT Rabbit anti-Human Polyclonal (aa517-732) Antibody reacts with Mouse, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHAT can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CHAT chat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHAT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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