Application Data
Rabbit Cofilin 1 (Ser-3) Polyclonal Antibody | anti-CFL1 antibody
Cofilin 1 (Ser-3), phospho-specific
Gene Names
CFL1; CFL
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Purity
Affinity Purified
Synonyms
Cofilin 1 (Ser-3), Antibody; Cofilin 1 (Ser-3), phospho-specific; p18; 18 kDa phosphoprotein; anti-CFL1 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to unphosphorylated Coflin 1 peptide then affinity purified using phospho-Cofilin 1 (Ser-3) peptide (without carrier). The antibody detects a 19 kDa* protein corresponding to the molecular mass of phosphorylated Cofilin 1 on SDS-PAGE immunoblots of Jurkat cells. This band can be removed by lambda phosphatase treatment.
Purity/Purification
Affinity Purified
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Sequence Length
166
Applicable Applications for anti-CFL1 antibody
WB (Western Blot), ELISA
Immunogen
A synthetic phospho-peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 3 in human Cofilin 1. This sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has two amino acid differences from human Cofilin 2.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Application Data
Application Data
Application Data
Application Data
Related Product Information for anti-CFL1 antibody
Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such as cell migration, cytokinesis, phagocytosis, endocytosis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (Cofilin 2), non-muscle cofilin (Cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major amino acid differences found in the C-terminal region. Regulation of cofilin activity can occur through serine phosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, PP1, PP2A, PP2B, slingshot, and chronophin can dephosphorylate Ser-3 and activate actin binding. Thus, Ser-3 phosphorylation is a major site for the regulation of cofilin activity.
References
• Bamburg, J.R. (1999). Annu Rev Cell Dev Biol. 15:185
• Maciver, S.K. & Hussey, P.J. (2002). Genome Biol. 3(5): 3007
• Maciver, S.K. & Hussey, P.J. (2002). Genome Biol. 3(5): 3007
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,502 Da
NCBI Official Full Name
Cofilin-1
NCBI Official Synonym Full Names
cofilin 1 (non-muscle)
NCBI Official Symbol
CFL1
NCBI Official Synonym Symbols
CFL
NCBI Protein Information
cofilin-1; p18; OTTHUMP00000233679; OTTHUMP00000233680; OTTHUMP00000233681; OTTHUMP00000233682; OTTHUMP00000233740; OTTHUMP00000233741; OTTHUMP00000233742; 18 kDa phosphoprotein; cofilin, non-muscle isoform
UniProt Protein Name
Cofilin-1
UniProt Gene Name
CFL1
UniProt Synonym Gene Names
CFL
UniProt Entry Name
COF1_HUMAN
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Product Notes
The CFL1 cfl1 (Catalog #AAA71517) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cofilin 1 (Ser-3), phospho-specific reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cofilin 1 (Ser-3) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CFL1 cfl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cofilin 1 (Ser-3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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