WB (Western Blot)
(Detection of human CTCF by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, MCF-7, Hep-G2, A-549, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody (AAA211011 lot 7) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human, Mouse CTCF Polyclonal Antibody | anti-CTCF antibody
Rabbit anti-CTCF Antibody, Affinity Purified
Gene Names
CTCF; MRD21
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CTCF, Antibody; Rabbit anti-CTCF Antibody, Affinity Purified; CTCFL paralog; MRD21; CCCTC-binding factor (zinc finger protein); CCCTC-binding factor; 11-zinc finger protein; 11 zinc finger transcriptional repressor; transcriptional repressor CTCF; Transcriptional repressor CTCF; anti-CTCF antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-CTCF antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 677 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CTCF by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, MCF-7, Hep-G2, A-549, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody (AAA211011 lot 7) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human CTCF by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, MCF-7, Hep-G2, A-549, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody (AAA211011 lot 7) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of mouse CTCF by western blot. Samples: Whole cell lysate (50 ug) from CT26, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody (AAA211011 lot 7) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse CTCF by western blot. Samples: Whole cell lysate (50 ug) from CT26, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody (AAA211011 lot 7) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CTCF antibody (AAA211011-7 lot 7) used for IP at 6 ug per reaction. CTCF was also immunoprecipitated by a previous lot of this antibody (AAA211011 lot 6) and a second antibody against a different epitope of CTCF . For blotting immunoprecipitated CTCF, AAA211011 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CTCF antibody (AAA211011-7 lot 7) used for IP at 6 ug per reaction. CTCF was also immunoprecipitated by a previous lot of this antibody (AAA211011 lot 6) and a second antibody against a different epitope of CTCF . For blotting immunoprecipitated CTCF, AAA211011 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-CTCF antibody
CTCF is a versatile 11-Zn-finger transcription factor that may function as a repressor of transcription, activator transcription, or regulator of genomic imprinting. CTCF is a candidate tumor suppressor associated with invasive breast cancer, prostate cancer, and Wilm's tumor.
Product Categories/Family for anti-CTCF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,998 Da
NCBI Official Full Name
transcriptional repressor CTCF isoform 1
NCBI Official Synonym Full Names
CCCTC-binding factor (zinc finger protein)
NCBI Official Symbol
CTCF
NCBI Official Synonym Symbols
MRD21
NCBI Protein Information
transcriptional repressor CTCF; CTCFL paralog; 11-zinc finger protein; 11 zinc finger transcriptional repressor
UniProt Protein Name
Transcriptional repressor CTCF
UniProt Gene Name
CTCF
UniProt Entry Name
CTCF_HUMAN
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Product Notes
The CTCF ctcf (Catalog #AAA211011) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CTCF Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CTCF can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CTCF ctcf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTCF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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