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WB (Western Blot)
(Western blot analysis of extracts from HeLa cells (Lane 2), using DNA-PK antiobdy. The lane on the left is treated with systhesized peptide.)
Rabbit anti-Human DNA-PK Polyclonal Antibody | anti-DNA-PK antibody
DNA-PK Antibody
Gene Names
PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
DNA-PK, Antibody; DNA-PK Antibody; DNA-PKcs; DNA-dependent protein kinase catalytic subunit; DNPK1; EC 2.7.11.1; P460; anti-DNA-PK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total DNA-PK protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
4097
Applicable Applications for anti-DNA-PK antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from human DNA-PK.
Target Name
DNA-PK
Preparation and Storage
Store at -20 degree C
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells (Lane 2), using DNA-PK antiobdy. The lane on the left is treated with systhesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells (Lane 2), using DNA-PK antiobdy. The lane on the left is treated with systhesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells using DNA-PK antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells using DNA-PK antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using DNA-PK antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using DNA-PK antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using DNA-PK antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using DNA-PK antibody.)
Related Product Information for anti-DNA-PK antibody
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation.Wesley D. Block, Nucleic Acids Res., Apr 2004; 32: 1967 - 1972.Katherine S. Pawelczak, Nucleic Acids Res., Jan 2005; 33: 152 - 161.Eun-Jung Park, Nucleic Acids Res., Dec 2003; 31: 6819 - 6827.Suisheng Zhang, Nucleic Acids Res., Jan 2004; 32: 1 - 10.
Product Categories/Family for anti-DNA-PK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
465,501 Da
NCBI Official Full Name
DNA-dependent protein kinase catalytic subunit isoform 2
NCBI Official Synonym Full Names
protein kinase, DNA-activated, catalytic polypeptide
NCBI Official Symbol
PRKDC
NCBI Official Synonym Symbols
HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
NCBI Protein Information
DNA-dependent protein kinase catalytic subunit
UniProt Protein Name
DNA-dependent protein kinase catalytic subunit
UniProt Gene Name
PRKDC
UniProt Synonym Gene Names
HYRC; HYRC1; DNA-PK catalytic subunit; DNA-PKcs
UniProt Entry Name
PRKDC_HUMAN
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Product Notes
The DNA-PK prkdc (Catalog #AAA306324) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNA-PK Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNA-PK can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DNA-PK prkdc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA-PK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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