Rabbit DNase gamma Polyclonal Antibody | anti-DNasegamma antibody

DNase gamma

Cat. Num. AAA268986

• Reactivity: Human, Mouse, Rat, Pig, Horse, Rabbit
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Protein A affinity purified
• Synonyms: DNase gamma, Antibody; DNase gamma; Deoxyribonuclease gamma; Deoxyribonuclease I like 3; Deoxyribonuclease I like III; Deoxyribonuclease I-like 3; DHP2; DNAS1L3; DNase I homolog protein 2; DNase I homolog protein DHP2; DNase I like 3; DNase I-like 3; DNASE1L3; DNSL3_HUMAN; Liver and spleen DNase; LS DNase; LS-DNase; LSD; anti-DNasegamma antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Pig, Horse, Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A affinity purified
• Form/Format: 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-DNasegamma antibody: ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: KLH conjugated synthetic peptide derived from human DNase gamma 151-250/305
• Positive control: Liver and spleen, rat heart tissue.
• Subcellular location: Nucleus.
• Preparation and Storage: Store at -20°C for one year.; The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at 20°C.; When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4°C.; Avoid repeated freeze/thaw cycles.

WB (Western Blot)

(Sample:Liver (Mouse) Lysate at 40 ug. Spleen (Mouse) Lysate at 40 ug. Primary: Anti-DNase gamma at 1/300 dilution. Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5,000 dilution. Predicted band size: 36 kD. Observed band size: 32/36 kD.)

Application Data

(Tissue/ce l: rat heart tissue; 4% Paraformaldehyde-fixed and parafin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-DNase gamma Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, fo lowed by conjugation to the secondary antibody and DAB staining.)

Related Product Information for anti-DNasegamma antibody

Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA. Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis (PubMed:23229555, PubMed:24312463). The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (By similarity). Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity. Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).

NCBI and Uniprot Product Information

• UniProt Accession #: Q13609
• Molecular Weight: 36 KDa