Rabbit anti-Human E2F1 Polyclonal Antibody | anti-E2F1 antibody

E2F1 Antibody

Cat. Num. AAA231228

• Gene Names: E2F1; RBP3; E2F-1; RBAP1; RBBP3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: E2F1, Antibody; E2F1 Antibody; Transcription factor E2F1E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1; E2F1; RBBP3; anti-E2F1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 437

• Applicable Applications for anti-E2F1 antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Species: Human
• Immunogen: Recombinant human Transcription factor E2F1 protein
• Conjugate: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-22820 / sc-193
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IP (Immunoprecipitation)

(Immunoprecipitating E2F1 in 3T3 whole cell lysateLane 1: Rabbit control IgG (1ug)instead of AAA231228 in 3T3 whole cell lysate. For western blotting,a HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50000)Lane 2: AAA231228 (8ug)+ 3T3 whole cell lysate (500ug)Lane 3: 3T3 whole cell lysate (10ug))

IHC (Immunohistochemisry)

(Immunohistochemistry of paraffin-embedded human melanoma cancer using AAA231228 at dilution of 1:100)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human liver cancer using AAA231228 at dilution of 1:100)

WB (Western Blot)

(Western blotAll lanes: E2F1 antibody at 7 ug/ml+ 3T3 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 47 kDaObserved band size: 47 kDa)

Related Product Information for anti-E2F1 antibody

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812).

NCBI and Uniprot Product Information

• NCBI GI #: 12669911
• NCBI GeneID: 1869
• NCBI Accession #: NP_005216.1
• NCBI GenBank Nucleotide #: NM_005225.2
• UniProt Accession #: Q01094; Q13143; Q92768
• Molecular Weight: 46,920 Da
• NCBI Official Full Name: transcription factor E2F1
• NCBI Official Synonym Full Names: E2F transcription factor 1
• NCBI Official Symbol: E2F1
• NCBI Official Synonym Symbols: RBP3; E2F-1; RBAP1; RBBP3
• NCBI Protein Information: transcription factor E2F1
• UniProt Protein Name: Transcription factor E2F1
• UniProt Gene Name: E2F1
• UniProt Synonym Gene Names: RBBP3; E2F-1; RBAP-1; RBBP-3
• UniProt Entry Name: E2F1_HUMAN