WB (Western Blot)
(Western blot analysis of various lysates, using ECD antibody (AAA282409) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.)
Rabbit ECD Polyclonal Antibody | anti-ECD antibody
ECD Rabbit pAb
Gene Names
ECD; GCR2; HSGT1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
ECD, Antibody; ECD Rabbit pAb; GCR2; SGT1; HSGT1; anti-ECD antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Sequence
ESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKPTKN
Applicable Applications for anti-ECD antibody
WB (Western Blot)
Positive Samples
HeLa, Mouse brain, Rat brain
Cellular Location
cytoplasm, cytosol, nucleoplasm, nucleus
Research Area
Epigenetics Nuclear Signaling, Cancer, Signal Transduction, Endocrine Metabolism
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 435-644 of human ECD (NP_009196.1).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Ship: Ice Pack
Ship: Ice Pack
WB (Western Blot)
(Western blot analysis of various lysates, using ECD antibody (AAA282409) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of various lysates, using ECD antibody (AAA282409) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of HeLa, using ECD antibody (AAA282409) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of HeLa, using ECD antibody (AAA282409) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.)
Related Product Information for anti-ECD antibody
Enables histone acetyltransferase binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in cytosol and nucleoplasm.
Product Categories/Family for anti-ECD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
644
NCBI Official Full Name
Protein SGT1
NCBI Official Synonym Full Names
ecdysoneless homolog (Drosophila)
NCBI Official Symbol
ECD
NCBI Official Synonym Symbols
GCR2; HSGT1
NCBI Protein Information
protein SGT1; suppressor of GCR2; protein ecdysoneless homolog; suppressor of S. cerevisiae gcr2
UniProt Protein Name
Protein SGT1
UniProt Gene Name
ECD
UniProt Synonym Gene Names
SGT1; hSGT1
UniProt Entry Name
SGT1_HUMAN
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Product Notes
The ECD ecd (Catalog #AAA282409) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ECD Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ECD can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the ECD ecd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ESESVSKEEK EQNYDLTEVS ESMKAFISKV STHKGAELPR EPSEAPITFD ADSFLNYFDK ILGPRPNESD SDDLDDEDFE CLDSDDDLDF ETHEPGEEAS LKGTLDNLKS YMAQMDQELA HTCISKSFTT RNQVEPVSQT TDNNSDEEDS GTGESVMAPV DVDLNLVSNI LESYSSQAGL AGPASNLLQS MGVQLPDNTD HRPTSKPTKN. It is sometimes possible for the material contained within the vial of "ECD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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