WB (Western Blot)
(Detection of human and mouse EFTUD1 by western blot. Samples: Whole cell lysate (50 ug) from RKO, Jurkat, A-549, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EFTUD1 antibody (AAA213455 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human EFTUD1 Polyclonal Antibody | anti-EFTUD1 antibody
Rabbit anti-EFTUD1 Antibody, Affinity Purified
Gene Names
EFL1; RIA1; EFTUD1; FAM42A; HsT19294
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
EFTUD1, Antibody; Rabbit anti-EFTUD1 Antibody, Affinity Purified; EFTUD1; ribosome assembly 1 homolog; RIA1; HsT19294; FAM42A; elongation factor-like GTPase 1; elongation factor-like 1; elongation factor Tu GTP binding domain containing 1; EFL1; SDS2; elongation factor Tu GTP-binding domain-containing protein 1; Elongation factor-like GTPase 1; elongation factor like GTPase 1; anti-EFTUD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-EFTUD1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 700 and 750
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse EFTUD1 by western blot. Samples: Whole cell lysate (50 ug) from RKO, Jurkat, A-549, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EFTUD1 antibody (AAA213455 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse EFTUD1 by western blot. Samples: Whole cell lysate (50 ug) from RKO, Jurkat, A-549, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EFTUD1 antibody (AAA213455 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human EFTUD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EFTUD1 antibody (AAA213455 lot 1) used for IP at 1 ug per reaction. EFTUD1 was also immunoprecipitated by rabbit anti-EFTUD1 antibody For blotting immunoprecipitated EFTUD1, AAA213455 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human EFTUD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EFTUD1 antibody (AAA213455 lot 1) used for IP at 1 ug per reaction. EFTUD1 was also immunoprecipitated by rabbit anti-EFTUD1 antibody For blotting immunoprecipitated EFTUD1, AAA213455 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-EFTUD1 antibody
EFTUD1 is involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q7Z2Z2].
Product Categories/Family for anti-EFTUD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
119,887 Da
NCBI Official Full Name
elongation factor-like GTPase 1 isoform 1
NCBI Official Synonym Full Names
elongation factor like GTPase 1
NCBI Official Symbol
EFL1
NCBI Official Synonym Symbols
RIA1; EFTUD1; FAM42A; HsT19294
NCBI Protein Information
elongation factor-like GTPase 1
UniProt Protein Name
Elongation factor Tu GTP-binding domain-containing protein 1
UniProt Gene Name
EFTUD1
UniProt Synonym Gene Names
EFL1; FAM42A
UniProt Entry Name
ETUD1_HUMAN
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Product Notes
The EFTUD1 eftud1 (Catalog #AAA213455) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-EFTUD1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EFTUD1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the EFTUD1 eftud1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EFTUD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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