IF (Immunofluorescence)
(Fluorescent confocal image of MCF-7 cell stained with ESR1 isoform4 Antibody (Center)(AAA286121). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ESR1 isoform4 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ESR1 isoform4 immunoreactivity is localized to cytoplasm significantly.)
Rabbit anti-Human ESR1 isoform4 Polyclonal Antibody | anti-ESR1 antibody
ESR1 isoform4 Antibody (C-term)
Gene Names
ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Reactivity
Human
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ESR1 isoform4, Antibody; ESR1 isoform4 Antibody (C-term); ESR1; ESR; NR3A1; Estrogen receptor; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; anti-ESR1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ESR1 isoform4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-300 amino acids from the C-term region of human ESR1 isoform4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
271-300
Sequence Length
312
Applicable Applications for anti-ESR1 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of MCF-7 cell stained with ESR1 isoform4 Antibody (Center)(AAA286121). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ESR1 isoform4 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ESR1 isoform4 immunoreactivity is localized to cytoplasm significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of MCF-7 cell stained with ESR1 isoform4 Antibody (Center)(AAA286121). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ESR1 isoform4 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ESR1 isoform4 immunoreactivity is localized to cytoplasm significantly.)
WB (Western Blot)
(ESR1 isoform4 Antibody (Center) (AAA286121) western blot analysis in MCF-7cell line lysates (35ug/lane).This demonstrates the ESR1 isoform 4 antibody detected the ESR1 isoform 4 protein (arrow).)
WB (Western Blot)
(ESR1 isoform4 Antibody (Center) (AAA286121) western blot analysis in MCF-7cell line lysates (35ug/lane).This demonstrates the ESR1 isoform 4 antibody detected the ESR1 isoform 4 protein (arrow).)
Related Product Information for anti-ESR1 antibody
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.
References
Geradts, J., et al. Cancer Invest. 28(9):969-977(2010)
Hayes, D.F., et al. Clin. Pharmacol. Ther. 88(5):626-629(2010)
Lupien, M., et al. Genes Dev. 24(19):2219-2227(2010)
Corbo, R.M., et al. J. Gerontol. A Biol. Sci. Med. Sci. (2010) In press :
Kim, S., et al. Fertil. Steril. (2010) In press :
Hayes, D.F., et al. Clin. Pharmacol. Ther. 88(5):626-629(2010)
Lupien, M., et al. Genes Dev. 24(19):2219-2227(2010)
Corbo, R.M., et al. J. Gerontol. A Biol. Sci. Med. Sci. (2010) In press :
Kim, S., et al. Fertil. Steril. (2010) In press :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35.5K kDa
NCBI Official Full Name
estrogen receptor isoform 1
NCBI Official Synonym Full Names
estrogen receptor 1
NCBI Official Symbol
ESR1
NCBI Official Synonym Symbols
ER; ESR; Era; ESRA; ESTRR; NR3A1
NCBI Protein Information
estrogen receptor
UniProt Protein Name
Estrogen receptor
UniProt Gene Name
ESR1
UniProt Synonym Gene Names
ESR; NR3A1; ER
UniProt Entry Name
ESR1_HUMAN
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Product Notes
The ESR1 esr1 (Catalog #AAA286121) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 271-300. The ESR1 isoform4 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ESR1 isoform4 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ESR1 esr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ESR1 isoform4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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