ICC (Immunocytochemistry)
(Immunocytochemical labeling of Estrogen Receptor alpha in paraformaldehyde fixed and NP-40 permeabilized MDA-MB-231 cells. The cells were labeled with rabbit polyclonal anti-Estrogen Receptor alpha (EP5431). The antibody was detected using goat anti-rabbit DyLight 594.)
Rabbit Estrogen Receptor alpha Polyclonal Antibody | anti-ESR1 antibody
Estrogen Receptor alpha (C-terminus) Antibody
Gene Names
ESR1; ER; ESR; Era; ESRA; NR3A1
Reactivity
Human, Rat, Mouse
Applications
Immunocytochemistry, Immunoprecipitation, ELISA, Western Blot
Synonyms
Estrogen Receptor alpha, Antibody; Estrogen Receptor alpha (C-terminus) Antibody; Estrogen Receptor alpha (C-terminus); Estrogen Ra Cterm; anti-ESR1 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
The antibody was affinity purified using ERalpha (C-terminus) peptide (without carrier). This antibody detects several forms of ERalpha ranging from 66 to 35kDa* on SDS-PAGE immunoblots of MCF-7 cells treated with pervanadate, and MDA-MB-231 cells.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-ESR1 antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
ERalpha (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminus of human ERalpha. This sequence is well conserved in rat and mouse ERalpha, but is not found in ERbeta.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Estrogen Receptor alpha in paraformaldehyde fixed and NP-40 permeabilized MDA-MB-231 cells. The cells were labeled with rabbit polyclonal anti-Estrogen Receptor alpha (EP5431). The antibody was detected using goat anti-rabbit DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Estrogen Receptor alpha in paraformaldehyde fixed and NP-40 permeabilized MDA-MB-231 cells. The cells were labeled with rabbit polyclonal anti-Estrogen Receptor alpha (EP5431). The antibody was detected using goat anti-rabbit DyLight 594.)
WB (Western Blot)
(Western blot image of human MCF-7 cells treated with pervanadate (1mM) for 30 min. (lanes 1-6). Some lanes of the blot were then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with mouse monoclonal anti-ERalpha (Tyr-537) phospho-specific (lanes 1 & 2), rabbit polyclonal anti-ERalpha (C-terminus) (lanes 3 & 4), and rabbit polyclonal anti-ERalpha (Tyr-537) phospho-specific (lanes 5 & 6).)
WB (Western Blot)
(Western blot image of human MCF-7 cells treated with pervanadate (1mM) for 30 min. (lanes 1-6). Some lanes of the blot were then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with mouse monoclonal anti-ERalpha (Tyr-537) phospho-specific (lanes 1 & 2), rabbit polyclonal anti-ERalpha (C-terminus) (lanes 3 & 4), and rabbit polyclonal anti-ERalpha (Tyr-537) phospho-specific (lanes 5 & 6).)
Related Product Information for anti-ESR1 antibody
Estrogen receptor alpha (ERalpha) is a member of the steroid receptor superfamily and its structure includes an N-terminal ligand-independent transactivation domain (AF-1), a highly conserved DNA binding domain, and a C-terminal ligand-dependent transactivation domain (AF-2). AF-1 and AF-2 activate transcription independently and synergistically, and act in a promoter- and cell-specific manner. Phosphorylation at multiple sites provides an important mechanism to regulate ERalpha activity. Ser-104, Ser-106, Ser-118, and Ser-167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERalpha activity. In addition to these sites, phosphorylation of Tyr-537 has been implicated in maximal hormone binding, dimerization, and transcriptional activity. Tyr-537, located in the AF-2 domain, is phosphorylated by c-Src leading to nuclear export of ERalpha and degradation. Thus, a variety of phosphorylation events control ERalpha activity.
References
Anbalagan M, Rowan BG (2015) Mol Cell Endocrin. 418(3):264.
Castoria, G. et al. (2012) Oncogene. 31:4868.
Castoria, G. et al. (2012) Oncogene. 31:4868.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,216 Da
NCBI Official Full Name
estrogen receptor
NCBI Official Synonym Full Names
estrogen receptor 1
NCBI Official Symbol
ESR1
NCBI Official Synonym Symbols
ER; ESR; Era; ESRA; NR3A1
NCBI Protein Information
estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha delta 4 +49 isoform; nuclear receptor subfamily 3 group A member 1; estrogen
UniProt Protein Name
Estrogen receptor
UniProt Gene Name
ESR1
UniProt Synonym Gene Names
ESR; NR3A1; ER
UniProt Entry Name
ESR1_HUMAN
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Product Notes
The ESR1 esr1 (Catalog #AAA71636) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Estrogen Receptor alpha (C-terminus) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Estrogen Receptor alpha can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ESR1 esr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Estrogen Receptor alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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