ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation MCF7(1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-ESR1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 promoter.)
Rabbit anti-Human, Rat Estrogen receptor Polyclonal Antibody | anti-ESR1 antibody
Rabbit anti-human Estrogen receptor polyclonal Antibody
Gene Names
ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Reactivity
Human, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
>95%, Protein G purified
Synonyms
Estrogen receptor, Antibody; Rabbit anti-human Estrogen receptor polyclonal Antibody; Estrogen receptor (ER) (ER-alpha) (Estradiol receptor) (Nuclear receptor subfamily 3 group A member 1), ESR1, ESR NR3A1; anti-ESR1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Sequence Length
595
Applicable Applications for anti-ESR1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)
Application Notes
WB: 1:1000-1:5000
IHC: 1:20-1:200
IHC: 1:20-1:200
Immunogen
Recombinant Human Estrogen receptor protein (10-591AA)
Immunogen Species
Homo sapiens (Human)
Research Area
Signal Transduction
Target Names
ESR1
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation MCF7(1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-ESR1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 promoter.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation MCF7(1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-ESR1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 promoter.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA18813 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA18813 at dilution 1:100)
WB (Western Blot)
(All lanes: ESR1 antibody at 7ug/mlLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: Rat brain tissueLane 4: Colo320 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 67, 54, 48, 36 kDaObserved band size: 67 kDa)
WB (Western Blot)
(All lanes: ESR1 antibody at 7ug/mlLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: Rat brain tissueLane 4: Colo320 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 67, 54, 48, 36 kDaObserved band size: 67 kDa)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
estrogen receptor isoform 1
NCBI Official Synonym Full Names
estrogen receptor 1
NCBI Official Symbol
ESR1
NCBI Official Synonym Symbols
ER; ESR; Era; ESRA; ESTRR; NR3A1
NCBI Protein Information
estrogen receptor
UniProt Protein Name
Estrogen receptor
UniProt Gene Name
ESR1
UniProt Synonym Gene Names
ESR; NR3A1; ER
UniProt Entry Name
ESR1_HUMAN
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Product Notes
The ESR1 esr1 (Catalog #AAA18813) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Estrogen receptor polyclonal Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Estrogen receptor can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP). WB: 1:1000-1:5000 IHC: 1:20-1:200. Researchers should empirically determine the suitability of the ESR1 esr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Estrogen receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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