IHC (Immunohiostchemistry)
(Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (FRMD4A) Polyclonal Antibody, Unconjugated (bs-8235R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
Rabbit FRMD4A Polyclonal Antibody | anti-FRMD4A antibody
FRMD4A Polyclonal Antibody
Gene Names
FRMD4A; FRMD4; bA295P9.4
Reactivity
Rat, Dog, Cow
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FRMD4A, Antibody; FRMD4A Polyclonal Antibody; FERM domain-containing protein 4A; FRMD4A; FRMD4; KIAA1294; anti-FRMD4A antibody
Host
Rabbit
Reactivity
Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-FRMD4A antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (FRMD4A) Polyclonal Antibody, Unconjugated (bs-8235R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohiostchemistry)
(Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (FRMD4A) Polyclonal Antibody, Unconjugated (bs-8235R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
WB (Western Blot)
(Sample:K562(Human) Cell Lysate at 30 ugPrimary: Anti- FRMD4A (bs-8235R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 115 kDObserved band size: 115 kD)
WB (Western Blot)
(Sample:K562(Human) Cell Lysate at 30 ugPrimary: Anti- FRMD4A (bs-8235R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 115 kDObserved band size: 115 kD)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
115,458 Da
NCBI Official Full Name
FERM domain-containing protein 4A
NCBI Official Synonym Full Names
FERM domain containing 4A
NCBI Official Symbol
FRMD4A
NCBI Official Synonym Symbols
FRMD4; bA295P9.4
NCBI Protein Information
FERM domain-containing protein 4A
UniProt Protein Name
FERM domain-containing protein 4A
UniProt Gene Name
FRMD4A
UniProt Synonym Gene Names
FRMD4; KIAA1294
UniProt Entry Name
FRM4A_HUMAN
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Product Notes
The FRMD4A frmd4a (Catalog #AAA292133) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FRMD4A Polyclonal Antibody reacts with Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's FRMD4A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the FRMD4A frmd4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FRMD4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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