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Application Data
(Zhao, Shaorong et al. “Deciphering the performance of polo-like kinase 1 in triple-negative breast cancer progression according to the centromere protein U-phosphorylation pathway.” American journal of cancer research vol. 11,5 2142-2158. 15 May. 2021)
Rabbit GAPDH Polyclonal Antibody | anti-GAPDH antibody
GAPDH Rabbit Polyclonal Antibody
Gene Names
GAPDH; G3PD; GAPD
Reactivity
Human, Mouse, Rat, Rb, Ch, Mk, sheep, X
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Synonyms
GAPDH, Antibody; GAPDH Rabbit Polyclonal Antibody; GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH; YM3215; BH6842; anti-GAPDH antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Rb, Ch, Mk, sheep, X
Clonality
Polyclonal
Specificity
The antibody detects endogenous GAPDH protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Form/Format
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-GAPDH antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section)
Application Notes
WB: 1:5000
IHC: 1:200
IHC: 1:200
Immunogen
Recombinant Protein of GAPDH
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
Application Data
(Zhao, Shaorong et al. “Deciphering the performance of polo-like kinase 1 in triple-negative breast cancer progression according to the centromere protein U-phosphorylation pathway.” American journal of cancer research vol. 11,5 2142-2158. 15 May. 2021)
Application Data
(Zhao, Shaorong et al. “Deciphering the performance of polo-like kinase 1 in triple-negative breast cancer progression according to the centromere protein U-phosphorylation pathway.” American journal of cancer research vol. 11,5 2142-2158. 15 May. 2021)
Application Data
(Zhao, TingTing, et al. "Tangshen formula modulates gut Microbiota and reduces gut-derived toxins in diabetic nephropathy rats." Biomedicine & Pharmacotherapy 129 (2020): 110325.)
Application Data
(Zhao, TingTing, et al. "Tangshen formula modulates gut Microbiota and reduces gut-derived toxins in diabetic nephropathy rats." Biomedicine & Pharmacotherapy 129 (2020): 110325.)
Application Data
(Yi, Sheng, et al. "Peripheral nerve injury induces dynamic changes of tight junction components." Frontiers in physiology9 (2018): 1519.)
Application Data
(Yi, Sheng, et al. "Peripheral nerve injury induces dynamic changes of tight junction components." Frontiers in physiology9 (2018): 1519.)
Application Data
(Chang, Ying, et al. "Lentivirus-mediated knockdown of astrocyte elevated gene-1 inhibits growth and induces apoptosis through MAPK pathways in human retinoblastoma cells." PloS one 11.2 (2016): e0148763.)
Application Data
(Chang, Ying, et al. "Lentivirus-mediated knockdown of astrocyte elevated gene-1 inhibits growth and induces apoptosis through MAPK pathways in human retinoblastoma cells." PloS one 11.2 (2016): e0148763.)
Application Data
(Wang, Xiao, et al. "Knockdown of Phospholipase C? (PLC?) Inhibits Cell Proliferation via Phosphatase and Tensin Homolog Deleted on Chromosome 10 (PTEN)/AKT Signaling Pathway in Human Prostate Cancer." Medical science monitor: international medical journal of experimental and clinical research 24 (2018): 254.)
Application Data
(Wang, Xiao, et al. "Knockdown of Phospholipase C? (PLC?) Inhibits Cell Proliferation via Phosphatase and Tensin Homolog Deleted on Chromosome 10 (PTEN)/AKT Signaling Pathway in Human Prostate Cancer." Medical science monitor: international medical journal of experimental and clinical research 24 (2018): 254.)
Application Data
(Wang, Chunhua, et al. "Salvianolic Acid B-Alleviated Angiotensin II Induces Cardiac Fibrosis by Suppressing NF-?B Pathway In Vitro." Medical science monitor: international medical journal of experimental and clinical research 24 (2018): 7654.)
Application Data
(Wang, Chunhua, et al. "Salvianolic Acid B-Alleviated Angiotensin II Induces Cardiac Fibrosis by Suppressing NF-?B Pathway In Vitro." Medical science monitor: international medical journal of experimental and clinical research 24 (2018): 7654.)
WB (Western Blot)
(Western blot analysis of 293T (1), Rat brain (2), NIH 3T3 (3), Sheep Muscle (4), Rabbit testis (5), diluted at 1:20000. Secondary antibody was diluted at 1:20000)
WB (Western Blot)
(Western blot analysis of 293T (1), Rat brain (2), NIH 3T3 (3), Sheep Muscle (4), Rabbit testis (5), diluted at 1:20000. Secondary antibody was diluted at 1:20000)
Related Product Information for anti-GAPDH antibody
glyceraldehyde-3-phosphate dehydrogenase (GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,053 Da
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase isoform 2
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_HUMAN
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Product Notes
The GAPDH gapdh (Catalog #AAA30771) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GAPDH Rabbit Polyclonal Antibody reacts with Human, Mouse, Rat, Rb, Ch, Mk, sheep, X and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section). WB: 1:5000 IHC: 1:200. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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