Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
Rabbit GRK2 Polyclonal Antibody | anti-GRK2 antibody
Phospho-GRK2 (Tyr86) Antibody
Gene Names
ADRBK1; GRK2; BARK1; BETA-ARK1
Reactivity
Human, Mouse, RatPredicted Reactivity: Bovine (100%), Horse (100%), Sheep (90%), Dog (100%), Chicken (90%)
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
GRK2, Antibody; Phospho-GRK2 (Tyr86) Antibody; ADRBK1; Adrenergic beta receptor kinase 1; ARBK1_HUMAN; BARK; BARK1; Beta adrenergic receptor kinase 1; Beta ARK 1; Beta ARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; FLJ16718; G protein coupled receptor kinase 2; G-protein coupled receptor kinase 2; GRK2; anti-GRK2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (90%), Dog (100%), Chicken (90%)
Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (90%), Dog (100%), Chicken (90%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-GRK2 (Tyr86) Antibody detects endogenous levels of GRK2 only when phosphorylated at Tyr86.
Tissue Specificity: Expressed in peripheral blood leukocytes.
Tissue Specificity: Expressed in peripheral blood leukocytes.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GRK2 antibody
ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human GRK2 around the phosphorylation site of Tyr86.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5. Interacts with ARRB1. Interacts with LPAR1 and LPAR2. Interacts with RALA in response to LPAR1 activation. ADRBK1 and RALA mutually inhibit each other's binding to LPAR1. Interacts with ADRB2.
Similarity
The pHdomain binds anionic phospholipids and helps recruiting ADRBK1 from the cytoplasm to plasma membrane close to activated receptors. It mediates binding to G protein beta and gamma subunits, competing with G-alpha subunits and other G-betagamma effectors.Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Subcellular Location
Cytoplasm. Cell membrane.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis GRK2 (Phospho-Tyr86) using UV treated 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis GRK2 (Phospho-Tyr86) using UV treated 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Related Product Information for anti-GRK2 antibody
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79,574 Da
NCBI Official Full Name
beta-adrenergic receptor kinase 1
NCBI Official Synonym Full Names
adrenergic, beta, receptor kinase 1
NCBI Official Symbol
ADRBK1
NCBI Official Synonym Symbols
GRK2; BARK1; BETA-ARK1
NCBI Protein Information
beta-adrenergic receptor kinase 1; beta-ARK-1; G-protein coupled receptor kinase 2
UniProt Protein Name
Beta-adrenergic receptor kinase 1
UniProt Gene Name
ADRBK1
UniProt Synonym Gene Names
BARK; BARK1; GRK2; Beta-ARK-1
UniProt Entry Name
ARBK1_HUMAN
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Product Notes
The GRK2 adrbk1 (Catalog #AAA329873) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-GRK2 (Tyr86) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (90%), Dog (100%), Chicken (90%) and may cross-react with other species as described in the data sheet. AAA Biotech's GRK2 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GRK2 adrbk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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