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WB (Western Blot)
(Anti-GSK3B rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa)
Rabbit anti-Mouse GSK-3 beta/GSK3B Polyclonal Antibody | anti-GSK3B antibody
Anti-GSK-3 beta/GSK3B Antibody, Rabbit Polyclonal
Reactivity
Mouse
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
GSK-3 beta/GSK3B, Antibody; Anti-GSK-3 beta/GSK3B Antibody, Rabbit Polyclonal; GSK-3 beta/GSK3B Antibody, Rabbit PAb, Antigen Affinity Purified; glycogen synthase kinase 3 beta; Anti-7330414F15Rik Antibody; Anti-8430431H08Rik Antibody; Anti-GSK-3 Antibody; Anti-GSK-3beta Antibody; Anti-GSK3 Antibody; Anti-Gsk3b Antibody; anti-GSK3B antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse GSK-3 beta/GSK3B
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GSK3B antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Mouse GSK-3 beta/GSK3B Protein ( Q9WV60; Met1-Thr420). GSK-3 beta/GSK3B specific IgG was purified by Mouse GSK-3 beta/GSK3B affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
WB (Western Blot)
(Anti-GSK3B rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa)
WB (Western Blot)
(Anti-GSK3B rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa)
IP (Immunoprecipitation)
(mGSK3B was immunoprecipitated using: Lane A:0.5 mg NIH/3T3 Whole Cell Lysate 4 uL anti-mGSK3B rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-mGSK3B rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa Observed band size :46 kDa)
IP (Immunoprecipitation)
(mGSK3B was immunoprecipitated using: Lane A:0.5 mg NIH/3T3 Whole Cell Lysate 4 uL anti-mGSK3B rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-mGSK3B rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa Observed band size :46 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of Mouse GSK3B in Mouse kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of Mouse GSK3B in Mouse kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of Mouse GSK3B in Mouse brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of Mouse GSK3B in Mouse brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-GSK3B antibody
GSK3B is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It Contains 1 protein kinase domain, and is expressed in the testis, thymus, prostate, and ovary and weakly expressed in the lung, brain, and kidney. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in the GSK3B gene have been implicated in modifying the risk of Parkinson's disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer's disease. GSK3B participates in the Wnt signaling pathway. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB, and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. GSK3B also plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. GSK3B phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which are critical for its role in bulge stem cell migration and skin wound repair. It may be required for early embryo development and neuron differentiation.
Product Categories/Family for anti-GSK3B antibody
References
Bergman. C. e. al. (2011. Inhibitio. o. glycoge. synthas. kinas. 3bet. induce. derma. fibrosi. b. activatio. o. th. canonica. Wn. pathway. An. Rheu. Dis. 70(12):2191-8.Ba. JO. e. al. (2011. Troglitazone. . PPA. agonist. inhibit. huma. prostat. cance. cel. growt. throug. inactivatio. o. NFkappa. vi. suppressio. o. GSK-3bet. expression. Cance. Bio. Ther. 12(4):288-96.Tsukig. M. e. al. (2012. Re-expressio. o. miR-199. suppresse. rena. cance. cel. proliferatio. an. surviva. b. targetin. GSK-3beta. Cance. Lett. 315(2):189-97.Nanda. D. e. al. (2012. Myeloi. cel. IL-1. productio. i. respons. t. leishmani. involve. inactivatio. o. glycoge. synthas. kinase-3bet. downstrea. o. phosphatidylinositol-. kinase. . Immunol. 188(1):367-78.
NCBI and Uniprot Product Information
UniProt Accession #
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Product Notes
The GSK3B (Catalog #AAA259467) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GSK-3 beta/GSK3B Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GSK-3 beta/GSK3B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GSK3B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK-3 beta/GSK3B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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