Rabbit Gsk3b Polyclonal Antibody | anti-Gsk3b antibody

Mouse Gsk3b Antibody (N-term)

Cat. Num. AAA289317

• Gene Names: Gsk3b; GSK3; GSK-3; C86142; GSK-3beta; 7330414F15Rik; 8430431H08Rik
• Reactivity: Mouse (Predicted Reactivity: Human, Rat)
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: Gsk3b, Antibody; Mouse Gsk3b Antibody (N-term); Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B; Gsk3b; anti-Gsk3b antibody

• Host: Rabbit
• Reactivity: Mouse (Predicted Reactivity: Human, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This Mouse Gsk3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of mouse Gsk3b.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 30-59
• Sequence Length: 420

• Applicable Applications for anti-Gsk3b antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Cellular Location: Cytoplasm {ECO:0000250|UniProtKB:P49841}. Nucleus {ECO:0000250|UniProtKB:P49841}. Cell membrane {ECO:0000250|UniProtKB:P49841}. Note=The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane (By similarity). {ECO:0000250|UniProtKB:P49841}.
• Tissue Location: Expressed in the liver (at protein level).
• Function: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1 (PubMed:15791206, PubMed:22057101, PubMed:23395175). Requires primed phosphorylation of the majority of its substrates (PubMed:15791206, PubMed:22057101, PubMed:23395175). In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis (By similarity). May also mediate the development of insulin resistance by regulating activation of transcription factors (By similarity). Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase (By similarity). In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes (By similarity). Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA (By similarity). Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin (By similarity). Phosphorylates MAPT/TAU on 'Thr- 548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules (By similarity). MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease (By similarity). Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (By similarity). Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair (PubMed:21295697). Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF- alpha (TNF/TNFA) (PubMed:10894547). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells and diabetes (PubMed:18288891). Through phosphorylation of the anti- apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation (PubMed:16543145). Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin (By similarity). Is necessary for the establishment of neuronal polarity and axon outgrowth (PubMed:17391670). Phosphorylates MARK2, leading to inhibition of its activity (By similarity). Phosphorylates SIK1 at 'Thr-182', leading to sustainment of its activity (By similarity). Phosphorylates ZC3HAV1 which enhances its antiviral activity (y similarity). Phosphorylates SNAI1, leading to its BTRC-triggered ubiquitination and proteasomal degradation (By similarity). Phosphorylates SFPQ at 'Thr-687' upon T-cell activation (By similarity). Phosphorylates NR1D1 st 'Ser-55' and 'Ser-59' and stabilizes it by protecting it from proteasomal degradation (By similarity). Regulates the circadian clock via phosphorylation of the major clock components including BMAL1, CLOCK and PER2 (PubMed:20049328, PubMed:28556462, PubMed:28903391, PubMed:20123978). Phosphorylates CLOCK AT 'Ser-427' and targets it for proteasomal degradation (By similarity). Phosphorylates BMAL1 at 'Ser-17' and 'Ser- 21' and primes it for ubiquitination and proteasomal degradation (PubMed:20049328, PubMed:28903391). Phosphorylates FBXL2 at 'Thr-404' and primes it for ubiquitination by the SCF(FBXO3) complex and proteasomal degradation (PubMed:23542741). Phosphorylates OGT at 'Ser- 3' or 'Ser-4' which positively regulates its activity (By similarity). Phosphorylates MYCN in neuroblastoma cells which may promote its degradation (By similarity). Regulates the circadian rhythmicity of hippocampal long-term potentiation and BMAL1 and PER2 expression (PubMed:28556462). Acts as a regulator of autophagy by mediating phosphorylation of KAT5/TIP60 under starvation conditions, activating KAT5/TIP60 acetyltransferase activity and promoting acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer (PubMed:22539723). Negatively regulates extrinsic apoptotic signaling pathway via death domain receptors (By similarity). Promotes the formation of an anti- apoptotic complex, made of DDX3X, BRIC2 and GSK3B, at death receptors, including TNFRSF10B (By similarity). The anti-apoptotic function is most effective with weak apoptotic signals and can be overcome by stronger stimulation (By similarity). Phosphorylates E2F1, promoting the interaction between E2F1 and USP11, stabilizing E2F1 and promoting its activity (By similarity). Phosphorylates FXR1, promoting FXR1 ubiquitination by the SCF(FBXO4) complex and FXR1 degradation by the proteasome (PubMed:26240334, PubMed:29142209).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(Mouse Gsk3b Antibody (N-term) (AAA289317)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Gsk3b Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Anti-Gsk3b Antibody (N-term) at 1:1000 dilution + mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Mouse Gsk3b Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Gsk3b antibody detected the Gsk3b protein (arrow).)

Related Product Information for anti-Gsk3b antibody

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. May phosphorylate MUC1 and decrease the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1 (By similarity).

Product Categories/Family for anti-Gsk3b antibody

Cancer; Cardiovascular; Cell Biology; Developmental Biology; Signal Transduction

References

Liu, Y., et al. Diabetologia 53(12):2600-2610(2010)
Graham, J.A., et al. J. Biol. Chem. 285(43):32852-32859(2010)
Ahmad, I., et al. Oncogene (2010) In press :
Asada, N., et al. J. Neurosci. 30(26):8852-8865(2010)
Carew, R.M., et al. BMC Dev. Biol. 10, 73 (2010) :

NCBI and Uniprot Product Information

• NCBI GI #: 9790077
• NCBI GeneID: 56637
• NCBI Accession #: NP_062801.1
• NCBI GenBank Nucleotide #: NM_019827.6
• UniProt Accession #: Q9WV60
• Molecular Weight: 46710
• NCBI Official Full Name: glycogen synthase kinase-3 beta
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: Gsk3b
• NCBI Official Synonym Symbols: GSK3; GSK-3; C86142; GSK-3beta; 7330414F15Rik; 8430431H08Rik
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• UniProt Gene Name: Gsk3b
• UniProt Synonym Gene Names: GSK-3 beta
• UniProt Entry Name: GSK3B_MOUSE