WB (Western Blot)
(HEK293 overexpressing human IRF5 and probed at 1ug/ml (mock transfection in lane B).)
Goat IRF5 Polyclonal Antibody | anti-IRF5 antibody
Goat anti-IRF5 Antibody
Gene Names
IRF5; SLEB10
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
IRF5, Antibody; Goat anti-IRF5 Antibody; IRF5; interferon regulatory factor 5; SLEB10; IRF5 antibody; interferon regulatory factor 5 antibody; SLEB10 antibody; anti-IRF5 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QGPWPMHPAGMQ
Sequence Length
147
Applicable Applications for anti-IRF5 antibody
ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-QGPWPMHPAGMQ, from the C Terminus of the protein sequence according to NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1.
Epitope
C Terminus
Note
This antibody is expected to recognize all four reported isoforms (NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1). Reported variants NP_001092100.1, NP_001092101.1, NP_001092098.1, NP_001092097.1 represent identical protein: Reported variants NP_116032.1, NP_001092100.1 represent identical protein:
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
WB (Western Blot)
(HEK293 overexpressing human IRF5 and probed at 1ug/ml (mock transfection in lane B).)
WB (Western Blot)
(HEK293 overexpressing human IRF5 and probed at 1ug/ml (mock transfection in lane B).)
WB (Western Blot)
((0.1ug/ml) staining of lysate of cell line A549 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
((0.1ug/ml) staining of lysate of cell line A549 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,020 Da
NCBI Official Synonym Full Names
interferon regulatory factor 5
NCBI Official Symbol
IRF5
NCBI Official Synonym Symbols
SLEB10
NCBI Protein Information
interferon regulatory factor 5
UniProt Protein Name
Interferon regulatory factor 5
UniProt Gene Name
IRF5
UniProt Synonym Gene Names
IRF-5
UniProt Entry Name
IRF5_HUMAN
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Product Notes
The IRF5 irf5 (Catalog #AAA61297) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-IRF5 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's IRF5 can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the IRF5 irf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QGPWPMHPAG MQ. It is sometimes possible for the material contained within the vial of "IRF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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