FCM/FACS (Flow Cytometry)
(IRF5 Antibody (N-term) (Cat. #AAA289673) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit IRF5 Polyclonal Antibody | anti-IRF5 antibody
IRF5 Antibody (N-term)
Gene Names
IRF5; SLEB10
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
IRF5, Antibody; IRF5 Antibody (N-term); Interferon regulatory factor 5; IRF-5; IRF5; anti-IRF5 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This IRF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the N-terminal region of human IRF5.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
81-108
Sequence Length
147
Applicable Applications for anti-IRF5 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Region
81-108
Function
Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PubMed: , PubMed: , PubMed: , PubMed: , PubMed: ). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (By similarity). Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88- dependent pathway (By similarity). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed: ).
Cellular Location
Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm:upon activation by the TLR adapter MYD88 and subsequent phosphorylation, translocates to the nucleus.
Preparation and Storage
Maintain refrigerated at 2°C to 8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(IRF5 Antibody (N-term) (Cat. #AAA289673) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(IRF5 Antibody (N-term) (Cat. #AAA289673) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of IRF5 Antibody (N-term) AAA289673 with A549 cell followed by Alexa Fluor??489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of IRF5 Antibody (N-term) AAA289673 with A549 cell followed by Alexa Fluor??489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Western blot analysis of anti-IRF5 Antibody (N-term) AAA289673 in Ramos cell line lysates (35ug/lane). IRF5 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-IRF5 Antibody (N-term) AAA289673 in Ramos cell line lysates (35ug/lane). IRF5 (arrow) was detected using the purified Pab.)
Related Product Information for anti-IRF5 antibody
IRF5 is a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats.
Product Categories/Family for anti-IRF5 antibody
References
Nordmark,G., Genes Immun. 10 (1), 68-76 (2009)
Kim,Y.J., J. Rheumatol. 35 (11), 2106-2118 (2008)
Kim,Y.J., J. Rheumatol. 35 (11), 2106-2118 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56044
NCBI Official Full Name
interferon regulatory factor 5 isoform b
NCBI Official Synonym Full Names
interferon regulatory factor 5
NCBI Official Symbol
IRF5
NCBI Official Synonym Symbols
SLEB10
NCBI Protein Information
interferon regulatory factor 5
UniProt Protein Name
Interferon regulatory factor 5
UniProt Gene Name
IRF5
UniProt Synonym Gene Names
IRF-5
UniProt Entry Name
IRF5_HUMAN
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Product Notes
The IRF5 irf5 (Catalog #AAA289673) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 81-108. The IRF5 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's IRF5 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the IRF5 irf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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