FCM/FACS (Flow Cytometry)
(ISWI Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ISWI Polyclonal Antibody | anti-SMARCA5 antibody
ISWI Antibody (C-term)
Gene Names
SMARCA5; ISWI; SNF2H; hISWI; hSNF2H; WCRF135
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ISWI, Antibody; ISWI Antibody (C-term); SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; 364-; Sucrose nonfermenting protein 2 homolog; hSNF2H; SMARCA5; SNF2H; WCRF135; anti-SMARCA5 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ISWI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 946-975 amino acids from the C-terminal region of human ISWI.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.47 (varies by lot)
Sequence Positions
946-975
Sequence Length
1,052
Applicable Applications for anti-SMARCA5 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ISWI Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ISWI Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(ISWI Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ISWI antibody detected the ISWI protein (arrow).)
WB (Western Blot)
(ISWI Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ISWI antibody detected the ISWI protein (arrow).)
Related Product Information for anti-SMARCA5 antibody
The protein encoded by this gene is a member of the
SWI/SNF family of proteins. Members of this family have helicase
and ATPase activities and are thought to regulate transcription of
certain genes by altering the chromatin structure around those
genes. The protein encoded by this gene is a component of the
chromatin remodeling and spacing factor RSF, a facilitator of the
transcription of class II genes by RNA polymerase II. The encoded
protein is similar in sequence to the Drosophila ISWI chromatin
remodeling protein.
SWI/SNF family of proteins. Members of this family have helicase
and ATPase activities and are thought to regulate transcription of
certain genes by altering the chromatin structure around those
genes. The protein encoded by this gene is a component of the
chromatin remodeling and spacing factor RSF, a facilitator of the
transcription of class II genes by RNA polymerase II. The encoded
protein is similar in sequence to the Drosophila ISWI chromatin
remodeling protein.
References
Goldman, J.A., et al. J. Biol. Chem. 285(7):4645-4651(2010)
He, X., et al. Biochemistry 47(27):7025-7033(2008)
Sheu, J.J., et al. Cancer Res. 68(11):4050-4057(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
He, X., et al. Biochemistry 47(27):7025-7033(2008)
Sheu, J.J., et al. Cancer Res. 68(11):4050-4057(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
121905
NCBI Official Full Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
NCBI Official Symbol
SMARCA5
NCBI Official Synonym Symbols
ISWI; SNF2H; hISWI; hSNF2H; WCRF135
NCBI Protein Information
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
UniProt Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
UniProt Gene Name
SMARCA5
UniProt Synonym Gene Names
SNF2H; WCRF135; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; hSNF2H
UniProt Entry Name
SMCA5_HUMAN
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Product Notes
The SMARCA5 smarca5 (Catalog #AAA288405) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 946-975. The ISWI Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's ISWI can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SMARCA5 smarca5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ISWI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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