WB (Western Blot)
(Detection of human SNF2h/ISWI/SMARCA5 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, MCF-7, HEK293T, Jurkat, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SNF2h/ISWI/SMARCA5 antibody AAA211279 (AAA211279 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human SNF2h/ISWI/SMARCA5 Polyclonal Antibody | anti-SNF2h/ISWI/SMARCA5 antibody
Rabbit anti-SNF2h/ISWI/SMARCA5 Antibody, Affinity Purified
Gene Names
SMARCA5; ISWI; SNF2H; hISWI; hSNF2H; WCRF135
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SNF2h/ISWI/SMARCA5, Antibody; Rabbit anti-SNF2h/ISWI/SMARCA5 Antibody, Affinity Purified; SNF2H; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; WCRF135; ISWI; hSNF2H; hISWI; sucrose nonfermenting protein 2 homolog; sucrose nonfermenting-like 5; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5; anti-SNF2h/ISWI/SMARCA5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-SNF2h/ISWI/SMARCA5 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 50 and 100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SNF2h/ISWI/SMARCA5 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, MCF-7, HEK293T, Jurkat, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SNF2h/ISWI/SMARCA5 antibody AAA211279 (AAA211279 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human SNF2h/ISWI/SMARCA5 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, MCF-7, HEK293T, Jurkat, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SNF2h/ISWI/SMARCA5 antibody AAA211279 (AAA211279 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SNF2h/ISWI/SMARCA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SNF2h/ISWI/SMARCA5 antibody (AAA211279 lot 2) used for IP at 6 ug per reaction. SNF2h/ISWI/SMARCA5 was also immunoprecipitated by a previous lot of this antibody (AAA211279 lot 1) and a second antibody against a different epitope of SNF2h/ISWI/SMARCA5 . For blotting immunoprecipitated SNF2h/ISWI/SMARCA5, AAA211279 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human SNF2h/ISWI/SMARCA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SNF2h/ISWI/SMARCA5 antibody (AAA211279 lot 2) used for IP at 6 ug per reaction. SNF2h/ISWI/SMARCA5 was also immunoprecipitated by a previous lot of this antibody (AAA211279 lot 1) and a second antibody against a different epitope of SNF2h/ISWI/SMARCA5 . For blotting immunoprecipitated SNF2h/ISWI/SMARCA5, AAA211279 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-SNF2h/ISWI/SMARCA5 antibody
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SNF2h/ISWI, also known as WCRF135, is the gene product of SMARCA5 that associates with WSTF (Williams Syndrome transcription factor) and several other nuclear proteins to mediate chromatin remodeling. SNF2h is also part of the NoRC (nucleolar remodeling complex).
Product Categories/Family for anti-SNF2h/ISWI/SMARCA5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
1052
NCBI Official Full Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
NCBI Official Symbol
SMARCA5
NCBI Official Synonym Symbols
ISWI; SNF2H; hISWI; hSNF2H; WCRF135
NCBI Protein Information
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; sucrose nonfermenting-like 5; sucrose nonfermenting protein 2 homolog; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5
UniProt Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
UniProt Gene Name
SMARCA5
UniProt Synonym Gene Names
SNF2H; WCRF135; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; hSNF2H
UniProt Entry Name
SMCA5_HUMAN
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Product Notes
The SNF2h/ISWI/SMARCA5 smarca5 (Catalog #AAA211279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SNF2h/ISWI/SMARCA5 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SNF2h/ISWI/SMARCA5 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SNF2h/ISWI/SMARCA5 smarca5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNF2h/ISWI/SMARCA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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