Rabbit ITGA4 Polyclonal Antibody | anti-ITGA4 antibody

ITGA4 Antibody

Cat. Num. AAA167315

• Gene Names: ITGA4; IA4; CD49D
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Antigen affinity purification
• Synonyms: ITGA4, Antibody; ITGA4 Antibody; ITGA4; CD49D; IA4; MGC90518; anti-ITGA4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 277

• Applicable Applications for anti-ITGA4 antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant protein of human ITGA4
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Application Data

WB (Western Blot)

(Western blot analysis of Jurkat cell lysate using ITGA4 antibody.)

Related Product Information for anti-ITGA4 antibody

Integrins are alpha/beta heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1, 2). The integrin family contains at least 18 alpha and 8 beta subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1, 2). A pair of important alpha4 integrins, alpha4beta1 and alpha4beta7, interact with VCAM-1, fibronectin, and MAdCAM-1 at cell adhesions (3). Gene knockout and antibody blocking research reveal that alpha4 integrins play important roles in embryonic liver and heart development and in fetal lymphocyte homing (4-6). Phosphorylation at Ser988 within the cytoplasmic tail of integrin alpha4 blocks binding to paxillin and promotes leading edge migration (7, 8). On SDS-PAGE, integrin alpha4 can migrate at several different apparent molecular sizes, a 150 kDa mature protein and a 140 kDa precursor protein (a 180 kDa protein also exists under mild non-reducing conditions) (9). Integrin alpha4 has a cleavage site at Arg558, which results in a small portion of the protein as either an 80 kDa N-terminal or 70 kDa C-terminal fragment (10).

NCBI and Uniprot Product Information

• NCBI GI #: 51330376
• NCBI GeneID: 3676
• Molecular Weight: 21,536 Da
• NCBI Official Full Name: ITGA4 protein
• NCBI Official Synonym Full Names: integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• NCBI Official Symbol: ITGA4
• NCBI Official Synonym Symbols: IA4; CD49D
• NCBI Protein Information: integrin alpha-4; 269C wild type; CD49 antigen-like family member D; VLA-4 subunit alpha; antigen CD49D, alpha-4 subunit of VLA-4 receptor; integrin alpha 4; integrin alpha-4 subunit; integrin alpha-IV; very late activation protein 4 receptor, alpha 4 subunit
• UniProt Protein Name: Integrin alpha-4
• UniProt Gene Name: ITGA4
• UniProt Synonym Gene Names: CD49D
• UniProt Entry Name: ITA4_HUMAN