Rabbit ITGA4 / VLA-4 / CD49d Polyclonal Antibody | anti-ITGA4 antibody

Rabbit Polyclonal to Human ITGA4 / VLA-4 / CD49d

Cat. Num. AAA51968

• Gene Names: ITGA4; IA4; CD49D
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Peptide Column Affinity Chromatography
• Synonyms: ITGA4 / VLA-4 / CD49d, Antibody; Rabbit Polyclonal to Human ITGA4 / VLA-4 / CD49d; Anti-ITGA4 / VLA-4 / CD49d Antibody (Internal) IHC-plus; ITGA4; 269C wild type; CD49D; IA4; Integrin alpha-4; Integrin alpha 4; Integrin alpha-4 subunit; Integrin alpha-IV; VLA-4; VLA-4 subunit alpha; CD49d antigen; Human ITGA4; CD49d; anti-ITGA4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two isoforms are known to exist. The immunogen to this antibody is within the Integrin alpha4 recombinant protein fragment and thus will recognize it.
• Purity/Purification: Peptide Column Affinity Chromatography
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1032

• Applicable Applications for anti-ITGA4 antibody: WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
• Immunogen Type: Synthetic peptide
• Immunogen Species: ITGA4 / VLA-4 / CD49d was raised against Human.
• Immunogen: ITGA4 / VLA-4 / CD49d antibody was raised against a 15 amino acid peptide from near the center of human Integrin alpha 4. The immunogen is located within aa220-270 of integrin alpha 4.
• Family/Subfamily: Integrin / not assigned-integrin
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C (3 months), Long term Storage: -20°C. Avoid repeated freeze/thaw cycles.

WB (Western Blot)

(Western blot of Integrin alpha 4 in rat spleen tissue lysate with Integrin alpha 4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)

IF (Immunofluorescence)

(Immunofluorescence of Integrin alpha 4 in Jurkat cells with Integrin alpha 4 antibody at 2 ug/ml.)

IHC (Immunohistochemistry)

(Anti-ITGA4 / Integrin Alpha 4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-ITGA4 antibody

Integrin alpha 4 antibody can be used for the detection of Integrin alpha 4 by Western blot at 1 ug/mL. Despite its predicted molecular weight, Integrin alpha4 will often run at higher molecular weight in SDS-PAGE. Antibody can also be used for immunoflourescence starting at 2 ug/mL. Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Product Categories/Family for anti-ITGA4 antibody

Family: Integrin

NCBI and Uniprot Product Information

• NCBI GI #: 67191027
• NCBI GeneID: 3676
• NCBI Accession #: NP_000876.3
• NCBI GenBank Nucleotide #: NM_000885.4
• UniProt Accession #: P13612; Q7Z4L6; D3DPG4; AAB25486
• NCBI Official Full Name: integrin alpha-4
• NCBI Official Synonym Full Names: integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• NCBI Official Symbol: ITGA4
• NCBI Official Synonym Symbols: IA4; CD49D
• NCBI Protein Information: integrin alpha-4; 269C wild type; integrin alpha 4; integrin alpha-IV; VLA-4 subunit alpha; integrin alpha-4 subunit; CD49 antigen-like family member D; antigen CD49D, alpha-4 subunit of VLA-4 receptor; very late activation protein 4 receptor, alpha 4 subunit
• UniProt Protein Name: Integrin alpha-4
• UniProt Gene Name: ITGA4
• UniProt Synonym Gene Names: CD49D
• UniProt Entry Name: ITA4_HUMAN