Rabbit JNK1/JNK2/JNK3 Polyclonal Antibody | anti-JNK1/JNK2/JNK3 antibody

JNK1/JNK2/JNK3 (phospho-Thr183/Tyr185) Antibody

Cat. Num. AAA305548

• Gene Names: MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: JNK1/JNK2/JNK3, Antibody; JNK1/JNK2/JNK3 (phospho-Thr183/Tyr185) Antibody; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; JNK-46; anti-JNK1/JNK2/JNK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of JNK1/JNK2/JNK3 only when phosphorylated at Thr183/Tyr185.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 427

• Applicable Applications for anti-JNK1/JNK2/JNK3 antibody: IF (Immunofluorescence), WB (Western Blot)
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Thr183/Tyr185 (M-M-T(p)-P-Y(p)- V - V) derived from Human JNK1/JNK2/JNK3.
• Modification: Phospho-Thr183/Tyr185
• Target Name: JNK1/JNK2/JNK3
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

WB (Western Blot)

(Western blot analysis of extracts from 293 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using JNK1/JNK2/JNK3 (phospho-Thr183/Tyr185) Antibody.)

IF (Immunofluorescence)

(Immunofluorescence staining of methanol-fixed Hela cells using JNK1/JNK2/JNK3(phospho-Thr183/Tyr185) Antibody.)

WB (Western Blot)

(Western blot analysis of extracts from C6 cells untreated or treated with anisomycin using JNK1/JNK2/JNK3(phospho-Thr183/Tyr185) Antibody.)

Related Product Information for anti-JNK1/JNK2/JNK3 antibody

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4). /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: a-1 and a-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 a-2, and JUND binds only weakly to it. /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity

Product Categories/Family for anti-JNK1/JNK2/JNK3 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 513788279
• NCBI GeneID: 5599
• NCBI Accession #: NP_001265476.1
• NCBI GenBank Nucleotide #: NM_001278547.1
• UniProt Accession #: P45983; Q15709; Q15712; Q15713; Q308M2; B5BTZ5; B7ZLV4; D3DX88; D3DX92
• Molecular Weight: 35,333 Da
• NCBI Official Full Name: mitogen-activated protein kinase 8 JNK1 beta2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8
• NCBI Official Symbol: MAPK8
• NCBI Official Synonym Symbols: JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
• NCBI Protein Information: mitogen-activated protein kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase 8
• UniProt Gene Name: MAPK8
• UniProt Synonym Gene Names: JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c
• UniProt Entry Name: MK08_HUMAN