IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug KDM1A antibody. Western blot was performed from the immunoprecipitate using KDM1A antibody at a dilition of 1:500.)
Rabbit KDM1A Polyclonal Antibody | anti-KDM1A antibody
KDM1A Polyclonal Antibody
Gene Names
KDM1A; AOF2; KDM1; LSD1; BHC110
Reactivity
Human, Mouse, Rat
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Synonyms
KDM1A, Antibody; KDM1A Polyclonal Antibody; KDM1; AOF2; BHC110; KIAA0601; LSD1; KDM1A; anti-KDM1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Concentration
1mg/ml (varies by lot)
Sequence Length
852
Applicable Applications for anti-KDM1A antibody
ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human KDM1A
Calculated Molecular Weight
95kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug KDM1A antibody. Western blot was performed from the immunoprecipitate using KDM1A antibody at a dilition of 1:500.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug KDM1A antibody. Western blot was performed from the immunoprecipitate using KDM1A antibody at a dilition of 1:500.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using KDM1A antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using KDM1A antibody.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using KDM1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using KDM1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)
Related Product Information for anti-KDM1A antibody
Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells (1,6,7). In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation (8). Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8).
Product Categories/Family for anti-KDM1A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
852
NCBI Official Full Name
Lysine-specific histone demethylase 1A
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 1A
NCBI Official Symbol
KDM1A
NCBI Official Synonym Symbols
AOF2; KDM1; LSD1; BHC110
NCBI Protein Information
lysine-specific histone demethylase 1A; lysine (K)-specific demethylase 1; BRAF35-HDAC complex protein BHC110; amine oxidase (flavin containing) domain 2; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; flavin-containing amine oxidase domain-containing protein 2
UniProt Protein Name
Lysine-specific histone demethylase 1A
UniProt Gene Name
KDM1A
UniProt Synonym Gene Names
AOF2; KDM1; KIAA0601; LSD1
UniProt Entry Name
KDM1A_HUMAN
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Product Notes
The KDM1A kdm1a (Catalog #AAA37152) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KDM1A Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KDM1A can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KDM1A kdm1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDM1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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