WB (Western Blot)
(LSD1 / KDM1A antibody (pAb) tested by Western blot Nuclear extract of HeLa cells (30 ug per lane) probed with LSD1 / KDM1A antibody (1:2,000 dilution).)
Rabbit LSD1/KDM1A Polyclonal Antibody | anti-LSD1/KDM1A antibody
LSD1/KDM1A antibody (pAb)
Reactivity
Human, Mouse, Wide Range Predicted
Applications
Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Synonyms
LSD1/KDM1A, Antibody; LSD1/KDM1A antibody (pAb); Lysine-specific demethylase 1; AOF2; KDM1A; BHC110; anti-LSD1/KDM1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Wide Range Predicted
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-LSD1/KDM1A antibody
WB (Western Blot), IP (Immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This antibody was raised against full-length recombinant human LSD1/KDM1A protein.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(LSD1 / KDM1A antibody (pAb) tested by Western blot Nuclear extract of HeLa cells (30 ug per lane) probed with LSD1 / KDM1A antibody (1:2,000 dilution).)
WB (Western Blot)
(LSD1 / KDM1A antibody (pAb) tested by Western blot Nuclear extract of HeLa cells (30 ug per lane) probed with LSD1 / KDM1A antibody (1:2,000 dilution).)
ChIP (Chromatin Immunoprecipitation)
(LSD1 / KDM1A antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a human small lung cancer cell line (treated with the LSD1 inhibitor, ORY-1001, 1nM for 96 hours) and 5 ul of LSD1 / KDM1A antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
ChIP (Chromatin Immunoprecipitation)
(LSD1 / KDM1A antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a human small lung cancer cell line (treated with the LSD1 inhibitor, ORY-1001, 1nM for 96 hours) and 5 ul of LSD1 / KDM1A antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Related Product Information for anti-LSD1/KDM1A antibody
Short Description: Our LSD1/KDM1A antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Immunoprecipitation and Western blot; it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
Background: LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3K4me1 and Histone H3K4me2, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3K9me1 and Histone H3K9me2, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase.
Background: LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3K4me1 and Histone H3K4me2, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3K9me1 and Histone H3K9me2, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase.
Product Categories/Family for anti-LSD1/KDM1A antibody
NCBI and Uniprot Product Information
Molecular Weight
110kDa
Similar Products
Product Notes
The LSD1/KDM1A (Catalog #AAA59845) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LSD1/KDM1A antibody (pAb) reacts with Human, Mouse, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's LSD1/KDM1A can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the LSD1/KDM1A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LSD1/KDM1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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