IHC (Immunohiostchemistry)
(Immunohistochemistry of LSD1 in human spleen tissue with LSD1 antibody at 5 μg/mL.)
Rabbit LSD1 Polyclonal Antibody | anti-KDM1A antibody
LSD1 Antibody
Gene Names
KDM1A; AOF2; CPRF; KDM1; LSD1; BHC110
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column.
Synonyms
LSD1, Antibody; LSD1 Antibody; Lysine (K)-specific demethylase 1 A; Lysine-specific histone demethylase; amine oxidase flavin containing domain protein 2; AOF2; anti-KDM1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
LSD1 antibody is human, mouse, and rat reactive. At least two isoforms of LSD1 are known to exist; this antibody detects both.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
876
Applicable Applications for anti-KDM1A antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
LSD1 antibody was raised against a 19 amino acid peptide near the amino terminus of human LSD1.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
A549 Cell Lysate
Conjugate
Unconjugated
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
IHC (Immunohiostchemistry)
(Immunohistochemistry of LSD1 in human spleen tissue with LSD1 antibody at 5 μg/mL.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of LSD1 in human spleen tissue with LSD1 antibody at 5 μg/mL.)
WB (Western Blot)
(Western blot analysis of LSD1 in A549 cell lysate with LSD1 antibody at 1 μg/ml.)
WB (Western Blot)
(Western blot analysis of LSD1 in A549 cell lysate with LSD1 antibody at 1 μg/ml.)
Related Product Information for anti-KDM1A antibody
Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction (1). Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2). LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation (3,4). Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3). LSD1-mediated histone demethylation thus may have significant effects on gene expression.
Product Categories/Family for anti-KDM1A antibody
References
Shi Y, Lan F, Matson C, et al. Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. Cell 2004; 119:941-53. Kouzarides T. Histone methylation in transcriptional control. Curr. Opin. Genet. Dev. 2002; 12:198-209. Shi YJ, Matson C, Lan F, et al. Regulation of LSD1 histone demethylase activity by its associated factors. Mol. Cell 2005; 19:857-64. Nakamura T, Mori T, Tada S, et al. ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation. Mol. Cell 2002; 10:1119-28.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 93, 96 kDa
Observed: 93, 96 kDa
Observed: 93, 96 kDa
NCBI Official Full Name
lysine-specific histone demethylase 1A isoform a
NCBI Official Synonym Full Names
lysine demethylase 1A
NCBI Official Symbol
KDM1A
NCBI Official Synonym Symbols
AOF2; CPRF; KDM1; LSD1; BHC110
NCBI Protein Information
lysine-specific histone demethylase 1A
UniProt Protein Name
Lysine-specific histone demethylase 1A
UniProt Gene Name
KDM1A
UniProt Synonym Gene Names
AOF2; KDM1; KIAA0601; LSD1
UniProt Entry Name
KDM1A_HUMAN
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Product Notes
The KDM1A kdm1a (Catalog #AAA40825) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LSD1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LSD1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the KDM1A kdm1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LSD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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