IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using [KO Validated] KEAP1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Rabbit KEAP1 Polyclonal Antibody | anti-KEAP1 antibody
[KO Validated] KEAP1 Rabbit pAb
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity purification
Synonyms
KEAP1, Antibody; [KO Validated] KEAP1 Rabbit pAb; KEAP1; INrf2; KLHL19; kelch-like ECH-associated protein 1; anti-KEAP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Sequence
MNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFKDLDFLQDIPDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPCVHLGRPDVLPQTPPLEEISEGSPIPDPSFSPLVGETLQMLTTSTTLEKLEVAESEFTH
Applicable Applications for anti-KEAP1 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Positive Samples
293T
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human KEAP1 (NP_036421.2).
Cellular Location
Cytoplasm, Nucleus
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using [KO Validated] KEAP1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using [KO Validated] KEAP1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] KEAP1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] KEAP1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and KEAP1 Rabbit pAb knockout (KO) 293T cells, using KEAP1 Rabbit pAb antibody at 1:1470 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and KEAP1 Rabbit pAb knockout (KO) 293T cells, using KEAP1 Rabbit pAb antibody at 1:1470 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Related Product Information for anti-KEAP1 antibody
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Product Categories/Family for anti-KEAP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,119 Da
NCBI Official Full Name
phosphatidylinositol 4-phosphate 5-kinase type-1 alpha isoform 4
NCBI Official Synonym Full Names
phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
NCBI Official Symbol
PIP5K1A
NCBI Protein Information
phosphatidylinositol 4-phosphate 5-kinase type-1 alpha; 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha; PIP5K1-alpha; PIP5KIalpha; phosphatidylinositol 4-phosphate 5-kinase type
UniProt Protein Name
Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
UniProt Gene Name
PIP5K1A
UniProt Synonym Gene Names
PIP5K1-alpha; PtdIns(4)P-5-kinase 1 alpha; PIP5KIalpha
UniProt Entry Name
PI51A_HUMAN
Similar Products
Product Notes
The KEAP1 pip5k1a (Catalog #AAA282196) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The [KO Validated] KEAP1 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KEAP1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the KEAP1 pip5k1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MNNLLPRSVK MHIKYDLKGS TYKRRASQKE REKPLPTFKD LDFLQDIPDG LFLDADMYNA LCKTLQRDCL VLQSFKIMDY SLLMSIHNID HAQREPLSSE TQYSVDTRRP APQKALYSTA MESIQGEARR GGTMETDDHM GGIPARNSKG ERLLLYIGII DILQSYRFVK KLEHSWKALV HDGDTVSVHR PGFYAERFQR FMCNTVFKKI PCVHLGRPDV LPQTPPLEEI SEGSPIPDPS FSPLVGETLQ MLTTSTTLEK LEVAESEFTH. It is sometimes possible for the material contained within the vial of "KEAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.