IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with MEF2C Antibody (S387) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit MEF2C Polyclonal Antibody | anti-MEF2C antibody
MEF2C Antibody (S387)
Gene Names
MEF2C; DEL5q14.3; C5DELq14.3
Reactivity
Human (Predicted Reactivity: Pig)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MEF2C, Antibody; MEF2C Antibody (S387); Myocyte-specific enhancer factor 2C; MEF2C; anti-MEF2C antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Pig)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MEF2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-394 amino acids from human MEF2C.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.38 (varies by lot)
Sequence Positions
365-394
Sequence Length
463
Applicable Applications for anti-MEF2C antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with MEF2C Antibody (S387) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with MEF2C Antibody (S387) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with MEF2C Antibody (S387). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEF2C primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).MEF2C immunoreactivity is localized to vesicles and Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with MEF2C Antibody (S387). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEF2C primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).MEF2C immunoreactivity is localized to vesicles and Nucleus significantly.)
WB (Western Blot)
(MEF2C Antibody (pS387) western blot analysis in HL-60,K562 cell line lysates (35ug/lane).This demonstrates the MEF2C antibody detected the MEF2C protein (arrow).)
WB (Western Blot)
(MEF2C Antibody (pS387) western blot analysis in HL-60,K562 cell line lysates (35ug/lane).This demonstrates the MEF2C antibody detected the MEF2C protein (arrow).)
Related Product Information for anti-MEF2C antibody
MEF2C is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. This protein controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It may also be involved in neurogenesis and in the development of cortical architecture.
Product Categories/Family for anti-MEF2C antibody
References
Konig, S., et al., J. Biol. Chem. 279(27):28187-28196 (2004). Maeda, T., et al., J. Biol. Chem. 277(50):48889-48898 (2002). Maeda, T., et al., Biochem. Biophys. Res. Commun. 294(4):791-797 (2002). Janson, C.G., et al., Brain Res. Mol. Brain Res. 97(1):70-82 (2001). Krainc, D., et al., Genomics 29(3):809-811 (1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51221
NCBI Official Full Name
myocyte-specific enhancer factor 2C isoform 2
NCBI Official Synonym Full Names
myocyte enhancer factor 2C
NCBI Official Symbol
MEF2C
NCBI Official Synonym Symbols
DEL5q14.3; C5DELq14.3
NCBI Protein Information
myocyte-specific enhancer factor 2C
UniProt Protein Name
Myocyte-specific enhancer factor 2C
UniProt Gene Name
MEF2C
UniProt Entry Name
MEF2C_HUMAN
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Product Notes
The MEF2C mef2c (Catalog #AAA285364) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 365-394. The MEF2C Antibody (S387) reacts with Human (Predicted Reactivity: Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's MEF2C can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the MEF2C mef2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEF2C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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