IP (Immunoprecipitation)
(Detection of human Phospho-MEK1 (T286) by immunoprecipitation (IP). Samples: 50 ug HeLa whole cell lysate (WCL) from mock treated (-) or nocodazole treated (+) cells. 1 mg of HeLa WCL mock treated (-) or nocodazole treated (+) and immunoprecipitated; 20% of the IP was loaded. Antibodies: Affinity purified rabbit anti-phospho MEK1 (T286) AAA212237 was used for IP (6ug/1mg lysate). To detect total MEK1, Rabbit anti-MEK1 (BL8445) was used for western blot (WB) at 1 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.)
Rabbit anti-Human, Mouse MEK1 Polyclonal Antibody | anti-MEK1 antibody
Rabbit anti-Phospho MEK1 (T286) Antibody, Affinity Purified
Gene Names
MAP2K1; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
MEK1, Antibody; Rabbit anti-Phospho MEK1 (T286) Antibody, Affinity Purified; MEK1, Phospho (T286); MAPKK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1); PRKMK1; MKK1; MEK1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; MAP kinase kinase 1; ERK activator kinase 1; CFC3; dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1; mitogen-activated protein kinase kinase 1; anti-MEK1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MEK1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Rat, Rabbit, Chimpanzee
Immunogen
Surrounding threonine 286
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human Phospho-MEK1 (T286) by immunoprecipitation (IP). Samples: 50 ug HeLa whole cell lysate (WCL) from mock treated (-) or nocodazole treated (+) cells. 1 mg of HeLa WCL mock treated (-) or nocodazole treated (+) and immunoprecipitated; 20% of the IP was loaded. Antibodies: Affinity purified rabbit anti-phospho MEK1 (T286) AAA212237 was used for IP (6ug/1mg lysate). To detect total MEK1, Rabbit anti-MEK1 (BL8445) was used for western blot (WB) at 1 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Phospho-MEK1 (T286) by immunoprecipitation (IP). Samples: 50 ug HeLa whole cell lysate (WCL) from mock treated (-) or nocodazole treated (+) cells. 1 mg of HeLa WCL mock treated (-) or nocodazole treated (+) and immunoprecipitated; 20% of the IP was loaded. Antibodies: Affinity purified rabbit anti-phospho MEK1 (T286) AAA212237 was used for IP (6ug/1mg lysate). To detect total MEK1, Rabbit anti-MEK1 (BL8445) was used for western blot (WB) at 1 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.)
IHC (Immunohiostchemistry)
(Detection of human Phospho-MEK1 (T286) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-phospho MEK1 (T286) (Cat. No. AAA212237 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human Phospho-MEK1 (T286) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-phospho MEK1 (T286) (Cat. No. AAA212237 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse Phospho-MEK1 (T286) by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-phospho MEK1 (T286) (Cat. No. AAA212237 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse Phospho-MEK1 (T286) by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-phospho MEK1 (T286) (Cat. No. AAA212237 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-MEK1 antibody
MEK1 is an activator and kinase for MAP and ERK kinases. It is a serine/threonine kinase essential to the MAP kinase signal transduction pathway which is involved in a multitude of cellular processes such as proliferation, differentiation, gene activation, and development. MEK1 displays 85% identity with MEK2. Although MEK1 and MEK2 have been found to be functionally similar, they may serve non-redundant functions. Both are required for cellular proliferation and may play a role in tumorigenesis.
Product Categories/Family for anti-MEK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,439 Da
NCBI Official Full Name
dual specificity mitogen-activated protein kinase kinase 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 1
NCBI Official Symbol
MAP2K1
NCBI Official Synonym Symbols
MEK1; MKK1; MAPKK1; PRKMK1
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; ERK activator kinase 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 1
UniProt Gene Name
MAP2K1
UniProt Synonym Gene Names
MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1
UniProt Entry Name
MP2K1_HUMAN
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Product Notes
The MEK1 map2k1 (Catalog #AAA212237) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Phospho MEK1 (T286) Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MEK1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the MEK1 map2k1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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