MEK1 recombinant antibody

Rabbit anti-MEK1 Recombinant Monoclonal Antibody [BLR095G]

Cat. Num. AAA213573

• Gene Names: MAP2K1; MEK1; MKK1; MAPKK1; PRKMK1
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Purity: Purified
• Synonyms: MEK1, Recombinant Antibody; Rabbit anti-MEK1 Recombinant Monoclonal Antibody [BLR095G]; MAPKK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1); PRKMK1; MKK1; MEK1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; MAP kinase kinase 1; ERK activator kinase 1; CFC3; dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1; mitogen-activated protein kinase kinase 1; MEK1 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR095G
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 100ug/ml (varies by lot)

• Applicable Applications for MEK1 recombinant antibody: FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 1 and 50
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human MEK1 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Hep-G2, Jurkat, A-549, HEK293T, RKO, U2OS, and LNCaP cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MEK1 recombinant monoclonal antibody (AAA213573 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 .)

WB (Western Blot)

(Detection of mouse MEK1 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, CTLL-2, EL4, RenCa, NIH 3T3, TCMK-1, C2C12, Ba/F3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MEK1 recombinant monoclonal antibody (AAA213573 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 .)

IP (Immunoprecipitation)

(Detection of human MEK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MEK1 recombinant monoclonal antibody (AAA213573 lot 1) used for IP at 20 ul/mg lysate. MEK1 was also immunoprecipitated by rabbit anti-MEK1 recombinant monoclonal antibody . For blotting immunoprecipitated MEK1, AAA213573 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

FCM/FACS (Flow Cytometry)

(Detection of human MEK1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-MEK1 recombinant monoclonal (AAA213573 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Related Product Information for MEK1 recombinant antibody

MEK1 is an activator and kinase for MAP and ERK kinases. It is a serine/threonine kinase essential to the MAP kinase signal transduction pathway which is involved in a multitude of cellular processes such as proliferation, differentiation, gene activation, and development. MEK1 displays 85% identity with MEK2. Although MEK1 and MEK2 have been found to be functionally similar, they may serve non-redundant functions. Both are required for cellular proliferation and may play a role in tumorigenesis.

Product Categories/Family for MEK1 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 5579478
• NCBI GeneID: 5604
• NCBI Accession #: NP_002746.1
• NCBI GenBank Nucleotide #: NM_002755.3
• UniProt Accession #: Q02750
• Molecular Weight: 43,439 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 1
• NCBI Official Symbol: MAP2K1
• NCBI Official Synonym Symbols: MEK1; MKK1; MAPKK1; PRKMK1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; ERK activator kinase 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 1
• UniProt Gene Name: MAP2K1
• UniProt Synonym Gene Names: MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1
• UniProt Entry Name: MP2K1_HUMAN